Zebrafish heparin-binding neurotrophic factor enhances neurite outgrowth during its development

Ming Huang Chang, Chang Jen Huang, Sheng Ping L Hwang, I. Ching Lu, Chien Ming Lin, Tzong Fu Kuo, Chih Ming Chou

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)


Heparin-binding neurotrophic factor (HBNF) is a secreted heparin-binding protein containing highly basic and cysteine-rich amino acid residues. In this study, we cloned the full-length HBNF cDNA from zebrafish and determined its genomic structure by bioinformatics analysis. Zebrafish HBNF gene is composed of five exons and four introns spanning approximately 82kb. RT-PCR analysis revealed that zebrafish HBNF transcript was highly expressed in adult brain and intestine tissues while less in other tissues. During embryogenesis, zebrafish HBNF transcript was observed to be moderately expressed at earlier stages with a gradual decline. Higher expression level was observed after hatching and maintaining this level into adulthood. The overall amino acid sequence of zebrafish HBNF shows 60% identity to human HBNF, but with approximately 40% identity to other midkine proteins. Like mammalian homolog, zebrafish HBNF could induce significant neurite outgrowth in PC12 cells without NGF stimulation. In addition, zebrafish HBNF was able to enhance extensive neurite outgrowth in zebrafish embryos during embryogenesis. In summary, a feasible in vivo assay for neurite outgrowth was established in zebrafish.
頁(從 - 到)502-509
期刊Biochemical and Biophysical Research Communications
出版狀態已發佈 - 8月 20 2004

ASJC Scopus subject areas

  • 生物化學
  • 生物物理學
  • 分子生物學


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