Ventilator-induced injury augments interleukin-1β production and neutrophil sequestration in lipopolysaccharide-treated lungs

Shu Min Lin, Horng Chyuan Lin, Kang Yun Lee, Chien Da Huang, Chien Ying Liu, Chun Hua Wang, Han Pin Kuo

研究成果: 雜誌貢獻文章同行評審

19 引文 斯高帕斯(Scopus)


Mechanical ventilators are commonly used to support critically ill patients; however, inappropriate ventilator settings might initiate or augment lung injury. To determine whether a large tidal volume (Vt) augments inflammatory responses and neutrophil sequestration in the lungs of rats receiving intratracheal lipopolysaccharides (LPS). Rats received intratracheal instillation of LPS (0.5 mg/kg) followed by 4 h of mechanical ventilation (MV) at 60 strokes per min with a Vt of 10 mL/kg as control MV, or 30 strokes per min with a Vt of 20 mL/kg of body weight as high-volume MV (HMV). In addition, monoclonal antibodies against rat intercellular adhesion molecule 1 (ICAM-1) or immunoglobulin G (50 mg/kg) were administered 30 min before LPS instillation and MV. Our study demonstrates that HMV enhances pulmonary permeability and induces neutrophil recruitment into the alveolar space and pulmonary edema. Intratracheal instillation of LPS caused marked lung injury, neutrophil recruitment, and production of cytokines and chemokines. Combining LPS instillation and HMV synergistically upregulated interleukin 1β (IL-1β) production and neutrophil sequestration in lung tissues. The ICAM-1 expression in lung tissues was responsible for the synergistic effects of neutrophil sequestration. Synergistic upregulation of IL-1β production and neutrophil sequestration was attenuated by blocking ICAM-1 by neutralizing antibody pretreatment. High Vt MV in LPS-injured lung causes synergistic production of IL-1β and sequestration of neutrophil via ICAM-1-dependent effects.
頁(從 - 到)453-460
出版狀態已發佈 - 10月 2007

ASJC Scopus subject areas

  • 急診醫學
  • 重症監護和重症監護醫學


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