TY - JOUR
T1 - Using genipin to immobilize bone morphogenetic protein-2 on zirconia surface for enhancing cell adhesion and mineralization in dental implant applications
AU - Sun, Ying Sui
AU - Lin, Yu An
AU - Huang, Her Hsiung
N1 - Funding Information:
Funding: This research was funded by the Ministry of Science and Technology (MOST), Taiwan (MOST 105-2314-B-030-MY3; MOST 107-2321-B-038-007-MY3).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11
Y1 - 2020/11
N2 - Our objective in this study was to promote cell responses through the immobilization of bone morphogenetic protein-2 (BMP-2) on roughened zirconia (ZrO2) through using the natural cross-linker genipin in dental implant applications. Field emission scanning electron microscope, X-ray photoelectron spectroscopy, and attenuated total reflection-Fourier transform infrared spectroscopy were used to analyze the surface characterizations, including the topography, chemistry, and functional groups, respectively, of the test specimens. Human bone marrow mesenchymal stem cells (hMSCs) were used to detect cell responses (adhesion, proliferation, and mineralization). The surface characterizations analysis results revealed that genipin was effective in immobilizing BMP-2 on roughened zirconia surfaces. BMP-2 proved effective in promoting the adhesion and mineralization of hMSCs on roughened zirconia. The surface modification proposed has potential in zirconia dental implant applications.
AB - Our objective in this study was to promote cell responses through the immobilization of bone morphogenetic protein-2 (BMP-2) on roughened zirconia (ZrO2) through using the natural cross-linker genipin in dental implant applications. Field emission scanning electron microscope, X-ray photoelectron spectroscopy, and attenuated total reflection-Fourier transform infrared spectroscopy were used to analyze the surface characterizations, including the topography, chemistry, and functional groups, respectively, of the test specimens. Human bone marrow mesenchymal stem cells (hMSCs) were used to detect cell responses (adhesion, proliferation, and mineralization). The surface characterizations analysis results revealed that genipin was effective in immobilizing BMP-2 on roughened zirconia surfaces. BMP-2 proved effective in promoting the adhesion and mineralization of hMSCs on roughened zirconia. The surface modification proposed has potential in zirconia dental implant applications.
KW - Bone morphogenetic protein-2 (BMP-2)
KW - Cell adhesion
KW - Cell mineralization
KW - Human bone marrow mesenchymal stem cell
KW - Natural cross-linker genipin
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U2 - 10.3390/polym12112639
DO - 10.3390/polym12112639
M3 - Article
AN - SCOPUS:85095932742
SN - 2073-4360
VL - 12
SP - 1
EP - 10
JO - Polymers
JF - Polymers
IS - 11
M1 - 2639
ER -