@article{e2188a4fa5a2479bad154c82c9846800,
title = "Uropathogenic Escherichia coli causes cortical tubular necrotic cell death and the release of macrophage migration inhibitory factor",
abstract = "The macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, is deregulated in acute kidney injury (AKI) through an unknown mechanism. In the present study, we used a previously described mouse model of ascending urinary tract infection in which uropathogenic Escherichia coli (UPEC) were transurethrally inoculated to induce kidney infections. Here, we show that urinary MIF was upregulated during AKI while MIF was abundantly expressed in the renal cortical tubules and that UPEC infection caused a decrease in tubular MIF. Infections with UPEC in vitro caused MIF release in a cell type-dependent manner, which was independent of receptor-mediated internalization, signal transduction, and transcription. Indeed, UPEC infection-induced necrotic cell death in vitro and in vivo correlated with extracellular acidification and processed MIF secretion. These data suggest that MIF is released by necrotic renal cortical tubular cells during UPEC infection.",
keywords = "AKI, Cortical tubular cell, MIF, Necrotic cell death, UPEC",
author = "Ming-Yuan Hong and Chin-Chung Tseng and Chia-Chang Chuang and Chia-Ling Chen and Yu-Huei Lin and Chia-Yuan Hsieh and Yu-Tzu Chang and Hsing, {Chung Hsi} and Kwang-Yu Chang and Lin, {Chiou Feng}",
note = "Funding Information: This work was supported by Grants NCKUH-9902016, NCKUH-10003019, and NSC 99-2320-B-006-004-MY3 and NSC 101-2314-B-006-060 from the National Science Council, Taiwan. We thank the Immunobiology Core, Research Center of Clinical Medicine, National Cheng Kung University Hospital, for providing services, including training, technical support, and assistance with experimental design and data analysis through the Flow Cytometry Core facilities. We are also grateful to Shang-Chi Lee, MSc for providing statistical consulting services from the Biostatistics Consulting Center, Research Center of Clinical Medicine, National Cheng Kung University Hospital. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",
year = "2013",
month = mar,
doi = "10.1016/j.cyto.2013.01.010",
language = "English",
volume = "61",
pages = "945--952",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press",
number = "3",
}