TY - JOUR
T1 - Two photon polymerization-micromolding of polyethylene glycol-gentamicin sulfate microneedles
AU - Gittard, Shaun D.
AU - Ovsianikov, Aleksandr
AU - Akar, Hasan
AU - Chichkov, Boris
AU - Monteiro-Riviere, Nancy A.
AU - Stafslien, Shane
AU - Chisholm, Bret
AU - Shin, Chun Che
AU - Shih, Chun Ming
AU - Lin, Shing Jong
AU - Su, Yea Yang
AU - Narayan, Roger J.
PY - 2010/4
Y1 - 2010/4
N2 - The use of microneedles for transdermal drug delivery is limited due to the risk of infection associated with formation of channels through the stratum corneum layer of the epidermis. The risk of infection associated with use of microneedles may be reduced by imparting these devices with antimicrobial properties. In this study, a photopolymerization-micromolding technique was used to fabricate microneedle arrays from a photosensitive material containing polyethylene glycol 600 diacrylate, gentamicin sulfate, and a photoinitiator. Scanning electron microscopy indicated that the photopolymerization-micromolding process produced microneedle arrays that exhibited good microneedle-to- microneedle uniformity. An agar plating assay revealed that microneedles fabricated with polyethylene glycol 600 diacrylate containing 2mg mL -1 gentamicin sulfate inhibited growth of Staphylococcus aureus bacteria. Scanning electron microscopy revealed no platelet aggregation on the surfaces of platelet rich plasma-exposed undoped polyethylene glycol 600 diacrylate microneedles and gentamicin-doped polyethylene glycol 600 diacrylate microneedles. These efforts will enable wider adoption of microneedles for transdermal delivery of pharmacologic agents.
AB - The use of microneedles for transdermal drug delivery is limited due to the risk of infection associated with formation of channels through the stratum corneum layer of the epidermis. The risk of infection associated with use of microneedles may be reduced by imparting these devices with antimicrobial properties. In this study, a photopolymerization-micromolding technique was used to fabricate microneedle arrays from a photosensitive material containing polyethylene glycol 600 diacrylate, gentamicin sulfate, and a photoinitiator. Scanning electron microscopy indicated that the photopolymerization-micromolding process produced microneedle arrays that exhibited good microneedle-to- microneedle uniformity. An agar plating assay revealed that microneedles fabricated with polyethylene glycol 600 diacrylate containing 2mg mL -1 gentamicin sulfate inhibited growth of Staphylococcus aureus bacteria. Scanning electron microscopy revealed no platelet aggregation on the surfaces of platelet rich plasma-exposed undoped polyethylene glycol 600 diacrylate microneedles and gentamicin-doped polyethylene glycol 600 diacrylate microneedles. These efforts will enable wider adoption of microneedles for transdermal delivery of pharmacologic agents.
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U2 - 10.1002/adem.200980012
DO - 10.1002/adem.200980012
M3 - Article
AN - SCOPUS:77951698312
SN - 1438-1656
VL - 12
SP - B77-B82
JO - Advanced Engineering Materials
JF - Advanced Engineering Materials
IS - 4
ER -