Treatment of osteoarthritis with collagen-based scaffold: A porcine animal model with xenograft mesenchymal stem cells

Wo Jan Tseng, Shu Wei Huang, Chih Hsiang Fang, Lih Tao Hsu, Chih Yu Chen, Hsin Hsin Shen, Jenny Zwei Chieng Chang, Jui Sheng Sun, Feng Huei Lin

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12 引文 斯高帕斯(Scopus)


Summary. Objective. With the goal to explore a new approach to treat the early degenerative lesions of hyaline cartilage, we implanted in a porcine OA model a collagen-based scaffold containing chondroprogenitor cells derived from human bone marrow mesenchymal stem cells (hBM-MSCs). Experimental design. Porcine knee joints were subjected to anterior cruciate ligament (ACL) transection to surgically induce OA. After 4 months, the time necessary for the development of cartilage surface damage, animals were treated either wi t h t r ephi nat i on bone pl ug wr apped wi t h t he chondroprogeni c hBM-MSCs-embedded col l agen scaffold or microfractures alone. Histological and histomorphometric evaluations were performed at 5 months after surgery. Results. All animals subjected to ACL t r ansect i on showed ost eoar t hr i t i c changes including mild lateral femoral condyle or moderate medial femoral condyle ulcerations. After 14 days’ chondrogenic induction, hBM-MSCs seeded onto the scaffold showed expression of chondroprogeni tor markers such as SOX9 and COMP. At 5 months after the implantation, significant differences in the quality of the regenerated tissue were found between the hBM-MSCs-embedded scaffold group and the control group. Newly generat ed t i ssue was onl y observed at t he si t e of implantation with the hBM-MSCs-embedded scaffolds. Furthermore, histological examination of the generated tissue revealed evidence of cartilage-like tissue with lacuna formation. In contrast, fibrous layers or fissures were formed on the surface of the control knee joint. Conclusions. This study shows that xenogenic hBM-MSC derived chondroprogenitor scaffolds can generate new cartilage tissue in porcine articular cartilage and have the potential as a useful treatment option for osteoarthritis.
頁(從 - 到)1271-1286
期刊Histology and Histopathology
出版狀態已發佈 - 2019

ASJC Scopus subject areas

  • 病理學與法醫學
  • 組織學


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