TY - JOUR
T1 - Transcriptomic Profiling Unravels Novel Deregulated Gene Signatures Associated with Acute Myocardial Infarction
T2 - A Bioinformatics Approach
AU - Kumar, Sanjay
AU - Shih, Chun Ming
AU - Tsai, Lung Wen
AU - Dubey, Rajni
AU - Gupta, Deepika
AU - Chakraborty, Tanmoy
AU - Sharma, Naveen
AU - Singh, Abhishek Vikram
AU - Swarup, Vishnu
AU - Singh, Himanshu Narayan
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/12/9
Y1 - 2022/12/9
N2 - Acute myocardial infarction (AMI) is a severe disease with elevated morbidity and mortality rate worldwide. This is attributed to great losses of cardiomyocytes, which can trigger the alteration of gene expression patterns. Although several attempts have been made to assess the AMI biomarkers, to date their role in rescuing myocardial injury remains unclear. Therefore, the current study investigated three independent microarray-based gene expression datasets from AMI patients (n = 85) and their age–sex-matched healthy controls (n = 70), to identify novel gene signatures that might be involved in cardioprotection. The differentially expressed genes (DEGs) were analyzed using ‘GEO2R’, and weighted gene correlation network analysis (WGCNA) was performed to identify biomarkers/modules. We found 91 DEGs, of which the number of upregulated and downregulated genes were 22 and 5, respectively. Specifically, we found that the deregulated genes such as ADOR-A3, BMP6, VPS8, and GPx3, may be associated with AMI. WGCNA revealed four highly preserved modules among all datasets. The ‘Enrichr’ unveiled the presence of miR-660 and STAT1, which is known to affect AMI severity. Conclusively, these genes and miRNA might play a crucial role the rescue of cardiomyocytes from severe damage, which could be helpful in developing appropriate therapeutic strategies for the management of AMI.
AB - Acute myocardial infarction (AMI) is a severe disease with elevated morbidity and mortality rate worldwide. This is attributed to great losses of cardiomyocytes, which can trigger the alteration of gene expression patterns. Although several attempts have been made to assess the AMI biomarkers, to date their role in rescuing myocardial injury remains unclear. Therefore, the current study investigated three independent microarray-based gene expression datasets from AMI patients (n = 85) and their age–sex-matched healthy controls (n = 70), to identify novel gene signatures that might be involved in cardioprotection. The differentially expressed genes (DEGs) were analyzed using ‘GEO2R’, and weighted gene correlation network analysis (WGCNA) was performed to identify biomarkers/modules. We found 91 DEGs, of which the number of upregulated and downregulated genes were 22 and 5, respectively. Specifically, we found that the deregulated genes such as ADOR-A3, BMP6, VPS8, and GPx3, may be associated with AMI. WGCNA revealed four highly preserved modules among all datasets. The ‘Enrichr’ unveiled the presence of miR-660 and STAT1, which is known to affect AMI severity. Conclusively, these genes and miRNA might play a crucial role the rescue of cardiomyocytes from severe damage, which could be helpful in developing appropriate therapeutic strategies for the management of AMI.
KW - acute myocardial infarction
KW - cardiovascular disease
KW - differential expressed genes
KW - NOTCH1 signaling
KW - weighted gene correlation network analysis
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U2 - 10.3390/genes13122321
DO - 10.3390/genes13122321
M3 - Article
C2 - 36553589
AN - SCOPUS:85144570191
SN - 2073-4425
VL - 13
JO - Genes
JF - Genes
IS - 12
M1 - 2321
ER -