Topoisomerase II is a structural component of mitotic chromosome scaffolds

William C. Earnshaw, Brian Halligan, Carol A. Cooke, Margarete M.S. Heck, Leroy F. Liu

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648 引文 斯高帕斯(Scopus)


We have obtained a polyclonal antibody that recognizes a major polypeptide component of chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr 170,000. Indirect immunofluorescence and subcellular fractionation experiments confirm that it is present in both mitotic chromosomes and interphase nuclei. Two lines of evidence suggest that this protein is DNA topoisomerase II, an abundant nuclear enzyme that controls DNA topological states: anti-scaffold antibody inhibits the strand-passing activity of DNA topoisomerase II; and both anti-scaffold antibody and an independent antibody raised against purified bovine topoisomerase II recognize identical partial proteolysis fragments of the 170,000-mol-wt scaffold protein in immunoblots. Our results suggest that topoisomerase II may be an enzyme that is also a structural protein of interphase nuclei and mitotic chromosomes.

頁(從 - 到)1706-1715
期刊Journal of Cell Biology
出版狀態已發佈 - 5月 1 1985

ASJC Scopus subject areas

  • 細胞生物學


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