Topographical modulation of pluripotency and differentiation of human embryonic stem cells

P. Y. Wang; S. Khan; T. Nguyen; P. Kingshott; R. C. B. Wong

doi:10.1109/TNANO.2017.2763604

Topographical modulation of pluripotency and differentiation of human embryonic stem cells

P. Y. Wang, S. Khan, T. Nguyen, P. Kingshott, R. C. B. Wong

奈米醫學工程研究所

研究成果: 雜誌貢獻 › 文章 › 同行評審

8 引文斯高帕斯（Scopus）

摘要

Surface topography can have significant impact on the cell fate decisions of stem cells. Construction of a biomaterial that can mimic the physiological microenvironment can be used to enhance the maintenance or differentiation of stem cells. Here we manufacture two surfaces with monolayer binary colloidal crystals (BCCs) having different topographies, Si2PM and Si5PM, and examined their effect on maintenance and differentiation of human embryonic stem cells (hESCs). Our results demonstrated that BCCs can be coupled with vitronectin to maintain pluripotency of hESCs during culture, and with a gelatin coating to improve early definitive endoderm differentiation of hESCs using an embryonic body assay (i.e. $FOXA2$ up-regulation). BCCs can be used as novel cell culture substrates to support stem cell maintenance and differentiation.

原文	英語
頁（從 - 到）	1
頁數	1
期刊	IEEE Transactions on Nanotechnology
卷	PP
發行號	99
DOIs	https://doi.org/10.1109/TNANO.2017.2763604
出版狀態	已發佈 - 2017

Keywords

Coatings
Proteins
Stem cells
Substrates
Surface topography
Surface treatment
binary colloidal crystals
biomaterials
differentiation
human embryonic stem cells
pluripotency
topography

存取文件

10.1109/TNANO.2017.2763604

引用此

@article{53015e9ac71942a68e90aa0f1ce72741,

title = "Topographical modulation of pluripotency and differentiation of human embryonic stem cells",

abstract = "Surface topography can have significant impact on the cell fate decisions of stem cells. Construction of a biomaterial that can mimic the physiological microenvironment can be used to enhance the maintenance or differentiation of stem cells. Here we manufacture two surfaces with monolayer binary colloidal crystals (BCCs) having different topographies, Si2PM and Si5PM, and examined their effect on maintenance and differentiation of human embryonic stem cells (hESCs). Our results demonstrated that BCCs can be coupled with vitronectin to maintain pluripotency of hESCs during culture, and with a gelatin coating to improve early definitive endoderm differentiation of hESCs using an embryonic body assay (i.e. FOXA2 up-regulation). BCCs can be used as novel cell culture substrates to support stem cell maintenance and differentiation.",

keywords = "Coatings, Proteins, Stem cells, Substrates, Surface topography, Surface treatment, binary colloidal crystals, biomaterials, differentiation, human embryonic stem cells, pluripotency, topography",

author = "Wang, {P. Y.} and S. Khan and T. Nguyen and P. Kingshott and Wong, {R. C. B.}",

year = "2017",

doi = "10.1109/TNANO.2017.2763604",

language = "English",

volume = "PP",

pages = "1",

journal = "IEEE Transactions on Nanotechnology",

issn = "1536-125X",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

number = "99",

}

TY - JOUR

T1 - Topographical modulation of pluripotency and differentiation of human embryonic stem cells

AU - Wang, P. Y.

AU - Khan, S.

AU - Nguyen, T.

AU - Kingshott, P.

AU - Wong, R. C. B.

PY - 2017

Y1 - 2017

N2 - Surface topography can have significant impact on the cell fate decisions of stem cells. Construction of a biomaterial that can mimic the physiological microenvironment can be used to enhance the maintenance or differentiation of stem cells. Here we manufacture two surfaces with monolayer binary colloidal crystals (BCCs) having different topographies, Si2PM and Si5PM, and examined their effect on maintenance and differentiation of human embryonic stem cells (hESCs). Our results demonstrated that BCCs can be coupled with vitronectin to maintain pluripotency of hESCs during culture, and with a gelatin coating to improve early definitive endoderm differentiation of hESCs using an embryonic body assay (i.e. FOXA2 up-regulation). BCCs can be used as novel cell culture substrates to support stem cell maintenance and differentiation.

AB - Surface topography can have significant impact on the cell fate decisions of stem cells. Construction of a biomaterial that can mimic the physiological microenvironment can be used to enhance the maintenance or differentiation of stem cells. Here we manufacture two surfaces with monolayer binary colloidal crystals (BCCs) having different topographies, Si2PM and Si5PM, and examined their effect on maintenance and differentiation of human embryonic stem cells (hESCs). Our results demonstrated that BCCs can be coupled with vitronectin to maintain pluripotency of hESCs during culture, and with a gelatin coating to improve early definitive endoderm differentiation of hESCs using an embryonic body assay (i.e. FOXA2 up-regulation). BCCs can be used as novel cell culture substrates to support stem cell maintenance and differentiation.

KW - Coatings

KW - Proteins

KW - Stem cells

KW - Substrates

KW - Surface topography

KW - Surface treatment

KW - binary colloidal crystals

KW - biomaterials

KW - differentiation

KW - human embryonic stem cells

KW - pluripotency

KW - topography

U2 - 10.1109/TNANO.2017.2763604

DO - 10.1109/TNANO.2017.2763604

M3 - Article

SN - 1536-125X

VL - PP

SP - 1

JO - IEEE Transactions on Nanotechnology

JF - IEEE Transactions on Nanotechnology

IS - 99

ER -

Topographical modulation of pluripotency and differentiation of human embryonic stem cells

摘要

Keywords

存取文件

指紋

引用此