Three-dimensional structure of the basketweave Z-band in midshipman fish sonic muscle

Thomas Burgoyne, John M. Heumann, Edward P. Morris, Carlo Knupp, Jun Liu, Michael K. Reedy, Kenneth A. Taylor, Kuan Wang, Pradeep K. Luther

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)


Striated muscle enables movement in all animals by the contraction of myriads of sarcomeres joined end to end by the Z-bands. The contraction is due to tension generated in each sarcomere between overlapping arrays of actin and myosin filaments. At the Z-band, actin filaments from adjoining sarcomeres overlap and are cross-linked in a regular pattern mainly by the protein α-actinin. The Z-band is dynamic, reflected by the 2 regular patterns seen in transverse section electron micrographs; the so-called small-square and basketweave forms. Although these forms are attributed, respectively, to relaxed and actively contracting muscles, the basketweave form occurs in certain relaxed muscles as in the muscle studied here. We used electron tomography and subtomogram averaging to derive the 3D structure of the Z-band in the swimbladder sonic muscle of type I male plainfin midshipman fish (Porichthys notatus), into which we docked the crystallographic structures of actin and α-actinin. The α-actinin links run diagonally between connected pairs of antiparallel actin filaments and are oriented at an angle of about 25° away from the actin filament axes. The slightly curved and flattened structure of the α-actinin rod has a distinct fit into the map. The Z-band model provides a detailed understanding of the role of α-actinin in transmitting tension between actin filaments in adjoining sarcomeres.
頁(從 - 到)15534-15539
期刊Proceedings of the National Academy of Sciences of the United States of America
出版狀態已發佈 - 7月 30 2019

ASJC Scopus subject areas

  • 多學科


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