The SEPT12 complex is required for the establishment of a functional sperm head-tail junction

Yi Ru Shen, Han Yu Wang, Yung Chieh Tsai, Yung Che Kuo, Shang Rung Wu, Chia Yih Wang, Pao Lin Kuo

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)


The connecting pieces of the sperm neck link the flagellum and the sperm head, and they are important for initiating flagellar beating. The connecting pieces are important building blocks for the sperm neck; however, the mechanism of connecting piece assembly is poorly understood. In the present study, we explored the role of septins in sperm motility and found that Sept12D197N knock-in (KI) mice produce acephalic and immotile spermatozoa. Electron microscopy analysis showed defective connecting pieces in sperm from KI mice, indicating that SEPT12 is required for the establishment of connecting pieces. We also found that SEPT12 formed a complex with SEPT1, SEPT2, SEPT10 and SEPT11 at the sperm neck and that the D197N mutation disrupted the complex, suggesting that the SEPT12 complex is involved in the assembly of connecting pieces. Additionally, we found that SEPT12 interacted and colocalized with γ-tubulin in elongating spermatids, implying that SEPT12 and pericentriolar materials jointly contribute to the formation of connecting pieces. Collectively, our findings suggest that SEPT12 is required for the formation of striated columns, and the capitulum and for maintaining the stability of the sperm head-tail junction.

頁(從 - 到)402-412
期刊Molecular Human Reproduction
出版狀態已發佈 - 6月 1 2020

ASJC Scopus subject areas

  • 生殖醫學
  • 胚胎學
  • 分子生物學
  • 遺傳學
  • 婦產科
  • 發展生物學
  • 細胞生物學


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