The role of the calmodulin-dependent pathway in static magnetic field-induced mechanotransduction

Jen Chang Yang, Sheng Yang Lee, Chi An Chen, Che Tong Lin, Chang Chih Chen, Haw Ming Huang

研究成果: 雜誌貢獻文章同行評審

15 引文 斯高帕斯(Scopus)


While the effects of static magnetic fields (SMFs) on osteoblastic differentiation are well demonstrated, the mechanotransduction pathways of SMFs are still unclear. The aim of this study was to explore the role of calmodulin in the biophysical effects of SMFs on osteoblastic cells. MG63 cells were exposed to a 0.4 T SMF The expression of phosphodiesterase RNA in the cytoplasm was tested using real-time polymerase chain reaction. The differentiation of the cells was assessed by detecting changes in alkaline phosphatase activity. The role of calmodulin antagonist W-7 was used to evaluate alterations in osteoblastic proliferation and differentiation after the SMF simulations. Our results showed that SMF exposure increased alkaline phosphatase activity and phosphodiesterase 1C gene expression in MG63 cells. Addition of W-7 significantly inhibited the SMF-induced cellular response. We suggest that one possible mechanism by which SMFs affects osteoblastic maturation is through a calmodulin-dependent mechanotransduction pathway.
頁(從 - 到)255-261
出版狀態已發佈 - 5月 2010

ASJC Scopus subject areas

  • 生物物理學
  • 放射學、核子醫學和影像學
  • 生理學


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