TY - JOUR
T1 - The porcine sperm motility inhibitor is identical to β-microseminoprotein and is a competitive inhibitor of Na+,K+-ATPase
AU - Chao, Chih Fang
AU - Chiou, Shean Tai
AU - Jeng, Hellen
AU - Chang, Wen Chang
PY - 1996/1/17
Y1 - 1996/1/17
N2 - We have isolated a small porcine seminal protein called SMI-1. Our results from peptide sequence, amino acid composition and mass spectral analyses reveal that SMI-1 is identical to porcine β-microseminoprotein, a protein with unknown function. We also report here that this protein inhibits competitively the activity of Na+,K+-ATPase purified from porcine cerebral cortex in a dose-dependent manner. The inhibitory effect could be reversed by the addition of ATP. The half-maximal inhibition was achieved at an inhibitor concentration of 90 μM.
AB - We have isolated a small porcine seminal protein called SMI-1. Our results from peptide sequence, amino acid composition and mass spectral analyses reveal that SMI-1 is identical to porcine β-microseminoprotein, a protein with unknown function. We also report here that this protein inhibits competitively the activity of Na+,K+-ATPase purified from porcine cerebral cortex in a dose-dependent manner. The inhibitory effect could be reversed by the addition of ATP. The half-maximal inhibition was achieved at an inhibitor concentration of 90 μM.
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U2 - 10.1006/bbrc.1996.0110
DO - 10.1006/bbrc.1996.0110
M3 - Article
C2 - 8561806
AN - SCOPUS:0030068487
SN - 0006-291X
VL - 218
SP - 623
EP - 628
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -