@article{14685ace7c854065b130b3a0bf3fc8af,
title = "The involvement of MCT-1 oncoprotein in inducing mitotic catastrophe and nuclear abnormalities",
abstract = "Centrosome amplification and chromosome abnormality are frequently identified in neoplasia and tumorigenesis. However, the mechanisms underlying these defects remain unclear. Here, we identify that MCT-1 is a centrosomal oncoprotein involved in mitosis. Knockdown of MCT-1 protein results in intercellular bridging, chromosome miscongregation, cytokinesis delay and mitotic death. Introduction of MCT-1 oncogene into the p53 deficient cells (MCT-1-p53), the mitotic checkpoint kinases and proteins are deregulated synergistically. These biochemical alterations are accompanied with increased frequencies of cytokinesis failure, multi-nucleation and centrosome amplification in subsequent cell cycle. As a result, the incidences of polyploidy and aneuploidy are progressively induced by prolonged cell cultivation or further promoted by sustained spindle damage on MCT-1-p53 background. These data show that the oncoprotein perturbs centrosome structure and mitotic progression, which provide the molecular aspect of chromsomal abnormality in vitro and the information for understanding the stepwise progression of tumors under oncogenic stress.",
keywords = "Centrosome, Cytokinesis, MCT-1, Mitosis, Nuclear abnormalities, p53",
author = "Shih, {Hung Ju} and Chu, {Kang Lin} and Wu, {Meng Hsun} and Wu, {Pei Hsuan} and Chang, {Wei Wen} and Chu, {Jan Show} and Wang, {Lily Hui Ching} and Hideki Takeuchi and Toru Ouchi and Hsu, {Hsin Ling}",
note = "Funding Information: deoxycholate, 1% NP40) supplemented with a protease inhibitor We thank Dr. Lu-Hai Wang for critical review of our manu-cocktail (Sigma). GST recombinant protein-coupled beads and script. This work was funded by MG-099-PP-05 (H.L.H.), 4 mg of lysates were mixed for 4 h at 4°C, washed with the lysis NSC96-2628-B-400-003-MY3 (H.L.H.), NSC99-buffer three times and then dissolved in the sample buffer before 3112-B-400-002 (H.L.H.), as well as partially supported by SDS-PAGE analysis. DOH100-TD-C-111-004 and Wan Fang Hospital (100swf14). Funding Information: MCF-10A cells were treated with nocodazole for 18 h, fol-This research was conducted under the Graduate Program of lowed by cultivation in complete medium for 1 h. Cells were Biotechnology in Medicine sponsored by the National Tsing Hua extracted with RIPA buffer (50 mM Tris-HCL pH 8.0, 150 mM University and the National Health Research Institutes.",
year = "2012",
month = mar,
day = "1",
doi = "10.4161/cc.11.5.19452",
language = "English",
volume = "11",
pages = "934--952",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Landes Bioscience",
number = "5",
}