@article{93135f379ac9403fa9a76c6b5e334c52,
title = "The Comparative Studies of Binding Activity of Curcumin and Didemethylated Curcumin with Selenite: Hydrogen Bonding vs Acid-Base Interactions",
abstract = "In this report, the in vitro relative capabilities of curcumin (CCM) and didemethylated curcumin (DCCM) in preventing the selenite-induced crystallin aggregation were investigated by turbidity tests and isothermal titration calorimetry (ITC). DCCM showed better activity than CCM. The conformers of CCM/SeO 3 2- and DCCM/SeO 3 2- complexes were optimized by molecular orbital calculations. Results reveal that the selenite anion surrounded by CCM through the H-bonding between CCM and selenite, which is also observed via IR and NMR studied. For DCCM, the primary driving force is the formation of an acid-base adduct with selenite showing that the phenolic OH group of DCCM was responsible for forming major conformer of DCCM. The formation mechanisms of selenite complexes with CCM or DCCM explain why DCCM has greater activity than CCM in extenuating the toxicity of selenite as to prevent selenite-induced lens protein aggregation.",
author = "Liao, {Jiahn Haur} and Wu, {Tzu Hua} and Chen, {Ming Yi} and Chen, {Wei Ting} and Lu, {Shou Yun} and Wang, {Yi Hsuan} and Wang, {Shao Pin} and Hsu, {Yen Min} and Huang, {Yi Shiang} and Huang, {Zih You} and Lin, {Yu Ching} and Chang, {Ching Ming} and Huang, {Fu Yung} and Wu, {Shih Hsiung}",
note = "Funding Information: We thank professor Shin-Guang Shyu and the staff at the Scientific Instrument Center at Academia Sinica for providing DFT software and assistance in using the Biophysics Core Facility instruments. This research is supported by grants from Academia Sinica and the National Science Council (NSC 100–2113-M-001–022-MY3; NSC 102–2811-M-001–132; NSC 98–2313-B-038-002-MY3; NSC 101– 2313-B-038-003; NSC 102–2313-B-038-005; MOST 103–2113-M-001 –029 -MY3, MOST 103–2811-M-001–126, MOST 104–2320-B-038 -018 ), Taipei Taiwan. We also received financial support from National Cheng Kung University, Tainan, Taiwan.",
year = "2015",
month = dec,
day = "4",
doi = "10.1038/srep17614",
language = "English",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}