TY - JOUR
T1 - The Clinical Experiences of Urine Metabolomics of Genitourinary Urothelial Cancer in a Tertiary Hospital in Taiwan
AU - Juang, Horng Heng
AU - Chen, Shao Ming
AU - Lin, Gigin
AU - Chiang, Meng Han
AU - Hou, Chen Pang
AU - Lin, Yu Hsiang
AU - Yang, Pei Shan
AU - Chang, Phei Lang
AU - Chen, Chien Lun
AU - Lin, Kuo Yen
AU - Tsui, Ke Hung
N1 - Funding Information:
The authors express thanks to the Metabolomics Core Laboratory, Healthy Aging Research Center (HARC), Chang Gung University, and Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital for the NMR spectroscopy analysis, supported by Chang Gung Memorial Hospital, Grant: CMRPG3H1321-2, CMRPG3K0821, CLRPG3K0021. Taiwan National Science Foundation grants NSC 107-2314-B-182A-017-MY3.
Publisher Copyright:
© Copyright © 2021 Juang, Chen, Lin, Chiang, Hou, Lin, Yang, Chang, Chen, Lin and Tsui.
PY - 2021/7
Y1 - 2021/7
N2 - Few studies have addressed the impact of diagnostic urine metabolites and the clinical outcomes associated with genitourinary urothelial (GU) cancer to date. Furthermore, longitudinal analysis of the dynamics of urine metabolites contributing to the detection of GU cancer has not yet been fully investigated; therefore, the discovery of novel diagnostic urine biomarkers is of enormous interest. We explored the correlation of the urine metabolomic profiles to GU cancers. The aqueous metabolites of the GU cancer and the control were also identified and analyzed through high-resolution1H nuclear magnetic resonance (NMR) spectroscopy. Compared with the control, the urine metabolites of the tumor were studied in relation to changes over time in a linear mixed model for repeated measures. The urine metabolites of sixty-three (44 male and 19 female) patients with GU cancers were systemically analyzed. The urine metabolite profile in GU cancer was significantly higher than those in the control group (p<0.05). Sevenurine metabolites including histidine, propylene glycol, valine, leucine, acetylsalicylate, glycine, and isoleucine as well as other pathways were identified statistically and were significantly associated with GU cancer detection with longitudinal analysis. We discovered that histidine, propylene glycol, valine, leucine, acetylsalicylate, glycine, isoleucine, succinic acid, lysine2-aminobutyric acid, and acetic acid are involved significantly in all types of male patients in whom the type (upper tract) of urine metabolites were found to be statistically significant compared with the control. We did not find any statistical significance in urine biomarkers between female and male patients. However, a statistically insignificant correlation was found among the grade and stage with the metabolites.
AB - Few studies have addressed the impact of diagnostic urine metabolites and the clinical outcomes associated with genitourinary urothelial (GU) cancer to date. Furthermore, longitudinal analysis of the dynamics of urine metabolites contributing to the detection of GU cancer has not yet been fully investigated; therefore, the discovery of novel diagnostic urine biomarkers is of enormous interest. We explored the correlation of the urine metabolomic profiles to GU cancers. The aqueous metabolites of the GU cancer and the control were also identified and analyzed through high-resolution1H nuclear magnetic resonance (NMR) spectroscopy. Compared with the control, the urine metabolites of the tumor were studied in relation to changes over time in a linear mixed model for repeated measures. The urine metabolites of sixty-three (44 male and 19 female) patients with GU cancers were systemically analyzed. The urine metabolite profile in GU cancer was significantly higher than those in the control group (p<0.05). Sevenurine metabolites including histidine, propylene glycol, valine, leucine, acetylsalicylate, glycine, and isoleucine as well as other pathways were identified statistically and were significantly associated with GU cancer detection with longitudinal analysis. We discovered that histidine, propylene glycol, valine, leucine, acetylsalicylate, glycine, isoleucine, succinic acid, lysine2-aminobutyric acid, and acetic acid are involved significantly in all types of male patients in whom the type (upper tract) of urine metabolites were found to be statistically significant compared with the control. We did not find any statistical significance in urine biomarkers between female and male patients. However, a statistically insignificant correlation was found among the grade and stage with the metabolites.
KW - bladder
KW - genitourinary urothelial cancer
KW - neoplasia
KW - urine metabolomics
KW - urothelium
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U2 - 10.3389/fonc.2021.680910
DO - 10.3389/fonc.2021.680910
M3 - Article
AN - SCOPUS:85112414182
SN - 2234-943X
VL - 11
JO - Frontiers in Oncology
JF - Frontiers in Oncology
M1 - 680910
ER -
