@article{52b95fb99edc4b178beadb2c7cb25c72,
title = "The application of non-invasive apoptosis detection sensor (NIADS) on histone deacetylation inhibitor (HDACI)-induced breast cancer cell death",
abstract = "Breast cancer is the most common malignancy in women and the second leading cause of cancer death in women. Triple negative breast cancer (TNBC) subtype is a breast cancer subset without ER (estrogen receptor), PR (progesterone receptor) and HER2 (human epidermal growth factor receptor 2) expression, limiting treatment options and presenting a poorer survival rate. Thus, we investigated whether histone deacetylation inhibitor (HDACi) could be used as potential anti-cancer therapy on breast cancer cells. In this study, we found TNBC and HER2-enriched breast cancers are extremely sensitive to Panobinostat, Belinostat of HDACi via experiments of cell viability assay, apoptotic marker identification and flow cytometry measurement. On the other hand, we developed a bioluminescence-based live cell non-invasive apoptosis detection sensor (NIADS) detection system to evaluate the quantitative and kinetic analyses of apoptotic cell death by HDAC treatment on breast cancer cells. In addition, the use of HDACi may also contribute a synergic anti-cancer effect with co-treatment of chemotherapeutic agent such as doxorubicin on TNBC cells (MDA-MB-231), but not in breast normal epithelia cells (MCF-10A), providing therapeutic benefits against breast tumor in the clinic.",
keywords = "HDACi, Live cell non-invasive apoptosis detection sensor (NIADS), Triple negative breast cancer (TNBC)",
author = "Hsu, {Kai Wen} and Huang, {Chien Yu} and Tam, {Ka Wai} and Lin, {Chun Yu} and Huang, {Li Chi} and Lin, {Ching Ling} and Hsieh, {Wen Shyang} and Chi, {Wei Ming} and Chang, {Yu Jia} and Wei, {Po Li} and Chen, {Shou Tung} and Lee, {Chia Hwa}",
note = "Funding Information: Acknowledgments: This study was supported China Medical University by grant CMU106-N-06 for Hsu, the Ministry of Science and Technology, grant 102-2320-B-038-039-MY3 for Lee, grant 104-2320-B-039-051 and 105-2320-B-039-010 for Hsu and the Health and Welfare Surcharge of Tobacco Products, Ministry of Health and Welfare to the Comprehensive Cancer Center of Taipei Medical University (MOHW105-TDU-B-212-134007, MOHW105-TDU-B-212-134001). Chun-Yu Lin acknowledges the JSPS International Research Fellowship. Funding Information: This study was supported China Medical University by grant CMU106-N-06 for Hsu, the Ministry of Science and Technology, grant 102-2320-B-038-039-MY3 for Lee, grant 104-2320-B-039-051 and 105-2320-B-039-010 for Hsu and the Health and Welfare Surcharge of Tobacco Products, Ministry of Health and Welfare to the Comprehensive Cancer Center of Taipei Medical University (MOHW105-TDU-B-212-134007, MOHW105-TDU-B-212-134001). Chun-Yu Lin acknowledges the JSPS International Research Fellowship. Publisher Copyright: {\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2018",
month = feb,
day = "2",
doi = "10.3390/ijms19020452",
language = "English",
volume = "19",
journal = "International journal of molecular sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "2",
}