@article{ca6689f9072741fe89b0b441a18804e6,
title = "The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB",
abstract = "The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.",
keywords = "Apoptosis, Ceramides, Lung cancer, NSCLC, cyclin A2, p-Akt, p-NFκB, survivin",
author = "Lin, {I. Ling} and Chou, {Han Lin} and Lee, {Jin Ching} and Chen, {Feng Wei} and Yao Fong and Chang, {Wei Chiao} and Huang, {Hurng W.} and Wu, {Chang Yi} and Chang, {Wen Tsan} and Wang, {Hui Min D.} and Chiu, {Chien Chih}",
note = "Funding Information: This study was financially supported by grants NSC101-2313-B-037-001, NSC101-2622-B-037-002-CC3 and NSC101-2320-B-037-046-MY3 from the National Science Council, Taiwan; by grant 101-CM-KMU-11 from ChiMei-KMU Joint Research Project; and by grant #NSYSUKMU 102–28 from the NSYSU-KMU Joint Research Project. The authors thank the help from the Statistical Analysis Laboratory, Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Taiwan.",
year = "2014",
month = jan,
day = "6",
doi = "10.1186/1475-2867-14-1",
language = "English",
volume = "14",
journal = "Cancer Cell International",
issn = "1475-2867",
publisher = "BioMed Central",
number = "1",
}
