Targeted methylation of two tumor suppressor genes is sufficient to transform mesenchymal stem cells into cancer stem/initiating cells

I. Wen Teng, Pei Chi Hou, Kuan Der Lee, Pei Yi Chu, Kun Tu Yeh, Victor X. Jin, Min Jen Tseng, Shaw Jenq Tsai, Yu Sun Chang, Chi Sheng Wu, H. Sunny Sun, Kuen Daw Tsai, Long Bin Jeng, Kenneth P. Nephew, Tim H.M. Huang, Shu Huei Hsiao, Yu Wei Leu

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89 引文 斯高帕斯(Scopus)

摘要

Although DNA hypermethylation within promoter CpG islands is highly correlated with tumorigenesis, it has not been established whether DNA hypermethylation within a specific tumor suppressor gene (TSG) is sufficient to fully transform a somatic stem cell. In this study, we addressed this question using a novel targeted DNA methylation technique to methylate the promoters of HIC1 and RassF1A, two well-established TSGs, along with a two-component reporter system to visualize successful targeting of human bone marrow-derived mesenchymal stem cells (MSC) as a model cell system. MSCs harboring targeted promoter methylations of HIC1/RassF1A displayed several features of cancer stem/initiating cells including loss of anchorage dependence, increased colony formation capability, drug resistance, and pluripotency. Notably, inoculation of immunodeficient mice with low numbers of targeted MSC resulted in tumor formation, and subsequent serial xenotransplantation and immunohistochemistry confirmed the presence of stem cell markers and MSC lineage in tumor xenografts. Consistent with the expected mechanism of TSG hypermethylation, treatment of the targeted MSC with a DNA methyltransferase inhibitor reversed their tumorigenic phenotype. To our knowledge, this is the first direct demonstration that aberrant TSG hypermethylation is sufficient to transform a somatic stem cell into a fully malignant cell with cancer stem/initiating properties.
原文英語
頁(從 - 到)4653-4663
頁數11
期刊Cancer Research
71
發行號13
DOIs
出版狀態已發佈 - 7月 1 2011
對外發佈

ASJC Scopus subject areas

  • 腫瘤科
  • 癌症研究

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