Tachycardia of atrial myocytes induces collagen expression in atrial fibroblasts through transforming growth factor β1

Chia Ti Tsai, Chuen Den Tseng, Juey Jen Hwang, Cho Kai Wu, Chih Chieh Yu, Yi Chih Wang, Wen Pin Chen, Ling Ping Lai, Fu Tien Chiang, Jiunn Lee Lin

研究成果: 雜誌貢獻文章同行評審

56 引文 斯高帕斯(Scopus)


Aims: We investigated the molecular mechanism of rapid-depolarization- induced atrial fibrosis. Methods and results: We used a direct atrial myocyte-fibroblast contact co-culture and a fibroblast-specific transforming growth factor β1 (TGF-β1), connective tissue growth factor (CTGF) and procollagen type I α-1 (COL1A1) luciferase reporter system to investigate the possible molecular mechanism of rapid-depolarization-induced atrial fibrosis. Mouse atrial fibroblasts were first transfected with promoter-luciferase reporters, and then co-cultured with HL-1 atrial myocytes. Rapid depolarization of atrial myocytes by rapid electrical field stimulation induced increased TGF-β1, CTGF and COL1A1 promoter activities in the co-cultured atrial fibroblasts (2.4 ± 0.3-fold increase, P = 0.008 for TGF-β1; 2.9 ± 0.4-fold increase, P < 0.001 for CTGF; and 2.1 ± 0.2-fold increase, P = 0.008 for COL1A1). Rapid depolarization of atrial myocytes increased paracrine secretion of angiotensin II (Ang II) and reactive oxygen species in the co-culture medium. Rapid electrical field stimulation-induced ROS generation in atrial myocytes was attenuated by the membrane NADPH oxidase inhibitor, apocynin. Atrial myocyte-induced expression of TGF-β1, CTGF and COL1A1 in atrial fibroblasts was attenuated by co-treatment with the Ang II receptor blocker, losartan, and apocynin. Atrial myocyte-induced COL1A1 expression in atrial fibroblasts was attenuated by anti-TGF-β1 antibody and RNA interference knockdown of the TGF-β1 receptor. Conclusion: We first demonstrated that tachycardia of atrial myocytes induced paracrine secretion of Ang II and reactive oxygen species, which in turn induced expression of CTGF and procollagen in co-cultured atrial fibroblasts through increasing TGF-β1 expression. The results may imply that use of an Ang II receptor blocker, in combination with an anti-oxidant, blocks rapid-depolarization-induced atrial fibrosis. Published on behalf of the European Society of Cardiology. All rights reserved.
頁(從 - 到)805-815
期刊Cardiovascular Research
出版狀態已發佈 - 3月 1 2011

ASJC Scopus subject areas

  • 心臟病學與心血管醫學
  • 生理學(醫學)
  • 生理學


深入研究「Tachycardia of atrial myocytes induces collagen expression in atrial fibroblasts through transforming growth factor β1」主題。共同形成了獨特的指紋。