TY - JOUR
T1 - Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane
AU - Lee, Shyh Yuan
AU - Wu, Sheng Chien
AU - Chen, Hsuan
AU - Tsai, Lo Lin
AU - Tzeng, Jy Jiunn
AU - Lin, Chih Hsin
AU - Lin, Yuan Min
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication of guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen and PTFE membranes. The PCL-based PUs were prepared by the polymerization of polycaprolactone (PCL) diol with 1,6-hexamethylene diisocyanate (HDI) at different ratios using either polyethylene glycol (PEG) or ethylenediamine (EDA) as chain extenders. The chemical, mechanical, and thermal properties of the synthesized polymers were determined using NMR, FTIR, GPC, DSC, and tensile tester. The PCL and polyurethanes were fabricated as nanofiber membranes by electrospinning, and their mechanical properties and SEM morphology were also investigated. In vitro tests, including WST-1 assay, SEM of cells, and phalloidin cytoskeleton staining, were also performed. It was shown that electrospun membranes made of PCL and PCL-HDI-PEG (2: 3: 1) possessed tensile strength of 19.84 MPa and 11.72 MPa and elongation at break of 627% and 362%, respectively. These numbers are equivalent or higher than most of the commercially available collagen and PTFE membrane. As a result, these membranes may have potential for future GBR applications.
AB - The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication of guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen and PTFE membranes. The PCL-based PUs were prepared by the polymerization of polycaprolactone (PCL) diol with 1,6-hexamethylene diisocyanate (HDI) at different ratios using either polyethylene glycol (PEG) or ethylenediamine (EDA) as chain extenders. The chemical, mechanical, and thermal properties of the synthesized polymers were determined using NMR, FTIR, GPC, DSC, and tensile tester. The PCL and polyurethanes were fabricated as nanofiber membranes by electrospinning, and their mechanical properties and SEM morphology were also investigated. In vitro tests, including WST-1 assay, SEM of cells, and phalloidin cytoskeleton staining, were also performed. It was shown that electrospun membranes made of PCL and PCL-HDI-PEG (2: 3: 1) possessed tensile strength of 19.84 MPa and 11.72 MPa and elongation at break of 627% and 362%, respectively. These numbers are equivalent or higher than most of the commercially available collagen and PTFE membrane. As a result, these membranes may have potential for future GBR applications.
KW - Animals
KW - Bone Regeneration
KW - Cell Line, Tumor
KW - Materials Testing
KW - Membranes, Artificial
KW - Mice
KW - Polyesters/chemical synthesis
KW - Polyurethanes/chemical synthesis
UR - http://www.scopus.com/inward/record.url?scp=85047883639&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047883639&partnerID=8YFLogxK
U2 - 10.1155/2018/3240571
DO - 10.1155/2018/3240571
M3 - Article
C2 - 29862262
AN - SCOPUS:85047883639
SN - 2314-6133
VL - 2018
SP - 3240571
JO - BioMed Research International
JF - BioMed Research International
M1 - 3240571
ER -