Suppression of cell growth by heavy chain ferritin

Jia Hsin Guo, Shu Hui Juan, Steven D. Aust

研究成果: 雜誌貢獻文章同行評審

11 引文 斯高帕斯(Scopus)


While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.

頁(從 - 到)39-45
期刊Biochemical and Biophysical Research Communications
出版狀態已發佈 - 1月 6 1998

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學


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