摘要
While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.
原文 | 英語 |
---|---|
頁(從 - 到) | 39-45 |
頁數 | 7 |
期刊 | Biochemical and Biophysical Research Communications |
卷 | 242 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 1月 6 1998 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 生物物理學
- 生物化學
- 分子生物學
- 細胞生物學