Study on cellar behaviors on different micro/nano structures of anodic aluminum oxide template

In this paper, we are investigating the different cultured structures scale in micrometer and nanometer. The experiment have been fabricated four types aluminum substrate structures which included none-structure, micro-structure, nano-structure, and combined micro/nano structure to fulfill the research in osteoblast-like cell (MG 63). Finally we performed the sterilization method to deal with the patterns of alumina sheet product in the clean room. Meanwhile, we demonstrated the osteoblast-like cell (MG63) to culture on the thin film. Finally, we have been used MTT assay study the behavior of cultured osteoblast-like cell to obtain the different properties between none-structure, micro-structure, nano-structure and micro/nano structure for adhesion, elongation and proliferation.