Study of bone marrow cells in non-Hodgkin's lymphoma by DNA analysis

Po min Chen, Sheng Fan, Siu Huie Lin, Cheng Hwai Tzeng, Jin Hwang Liu, Jacqueline M. Liu, Hsin mei Yeh, Tzeon Jye Chiou

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2 引文 斯高帕斯(Scopus)


Using Southern-blot analysis, we studied samples of bone marrow (BM) cells from 73 patients with non-Hodgkin's lymphoma (NHL) in various clinical status. The frequency of gene rearrangement was disease-status dependent with a frequency of 65.8% at the diagnostic stage, 81.8% after relapse and 33.3% upon complete remission (CR). BM involvement was evident in a substantial portion of patients with untreated and relapsed lymphoma. The significance of BM involvement by DNA hybridization in relation to conventional clinical staging and histological grade was studied. By Southern-blot analysis, BM involvement was found in 76% of the patients at clinical stages (CS) I-III. The incidence of BM involvement in low, intermediate and high grades of NHL (Working Formulation) was 57%( 4 7), 67%( 22 33), and 89%( 8 9) respectively. A comparative study of conventional BM biopsy vs DNA hybridization in a group of 47 NHL patients showed that all 12 patients (100%) with morphological BM involvement and 25 out of 35 patients (71%) with morphologically normal BM had clonal rearrangements of immunoglobulin (Ig), heavy chain and/or light chain, or T-cell receptor β chain (TCRβ) genes in BM cells. The false negative rate in conventional BM biopsy was 53%( 25 47). Southern-blot analysis on lymph nodes (LN) and BM cells from 37 patients showed that 6 patients (16%) had cross-lineage or different rearranged patterns in the same or different tissues. Southern-blot analysis was found to be highly reliable for the detection of even minimal populations of lymphoma cells in the BM and therefore should be the diagnostic choice for clinical staging of lymphoma.
頁(從 - 到)1097-1106
期刊Leukemia Research
出版狀態已發佈 - 1991

ASJC Scopus subject areas

  • 血液學
  • 腫瘤科
  • 癌症研究


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