Stimulation of osteogenic activity in human osteoblast cells by edible Uraria crinita

Yi Wen Mao, Rong Dih Lin, Hsiao Chiao Hung, Mei Hsien Lee

研究成果: 雜誌貢獻文章同行評審

17 引文 斯高帕斯(Scopus)


Uraria crinita is an edible herb used as a natural food for childhood skeletal dysplasia. Ethyl acetate, n-butanol, and aqueous fractions of a 95% ethanol crude extract of U. crinita were obtained and the active ingredients isolated and purified using a bioguided method. In this manner, we isolated and identified a new active flavone glycoside, apigenin 6-C-β-d- apiofuranosyl(1→2)-α-d-xylopyranoside (3) and 10 known components with stimulatory activity on human osteoblast cells. The new compound 3 at 100 μM significantly increased alkaline phosphatase activity (114.10 ± 4.41%), mineralization (150.10 ± 0.80%), as well as osteopontin (1.39 ± 0.01-fold), bone morphogenetic protein-2 (BMP-2, 1.30 ± 0.04-fold), and runt-related transcription factor 2 (Runx2, 1.43 ± 0.10-fold) mRNA expression through the activation of the BMP-2/Runx2 pathway. Two other components, dalbergioidin (1) and byzantionoside B (9), displayed similar effects. These results show that U. crinita and its active compounds may have the potential to stimulate bone formation and regeneration.

頁(從 - 到)5581-5588
期刊Journal of Agricultural and Food Chemistry
出版狀態已發佈 - 6月 18 2014

ASJC Scopus subject areas

  • 農業與生物科學 (全部)
  • 化學 (全部)
  • 醫藥 (全部)


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