TY - JOUR
T1 - Stimulation of interleukin-6 production by corticotropin-releasing factor
AU - Leu, Sy Jye Christine
AU - Singh, Vijendra K.
N1 - Funding Information:
This researchw as supported by grants from the Utah State University’s programs of Biotechnology Center Research and Faculty Research. Christine Leu is a Ph.D. student. The authors are most grateful to Dr. Otoniel Martinez-Maza of UCLA for the generous gift of MH60.BSF2 cell line, and to Professor Tadamitsu Kishimoto of Osaka University for his kind permission to obtain this cell line from Dr. Martinez-Maza.
PY - 1992/8
Y1 - 1992/8
N2 - Based on the immune-modulating properties of corticotropin-releasing factor (CRF), the effect of this peptide for interIeukin-6 (IL-6) production was investigated. Using human peripheral blood mononuclear cells (MNC), the amount of bioactive IL-6 produced was significantly (P ≤ 0.05) increased by CRF (10-10 to 10-7 M range). However, the IL-6 production of lipopolysac-charide-treated MNC cultures was not modified. At concentrations of ≥ 10 nM, CRF and two analogous peptides (Tyr-CRF and α-helical CRF) elicited 16- to 21-fold stimulation of IL-6 production by MNC. Purified monocytes, but not purified lymphocytes, were the cells that responded to CRF action exhibiting nearly 19-fold stimulation at 100 nM concentration. The CRF-induced production of IL-6 cytokine by peripheral blood MNC may suggest a messenger role for this neurohormone in the feedback control of neuroendocrine-immune circuitry.
AB - Based on the immune-modulating properties of corticotropin-releasing factor (CRF), the effect of this peptide for interIeukin-6 (IL-6) production was investigated. Using human peripheral blood mononuclear cells (MNC), the amount of bioactive IL-6 produced was significantly (P ≤ 0.05) increased by CRF (10-10 to 10-7 M range). However, the IL-6 production of lipopolysac-charide-treated MNC cultures was not modified. At concentrations of ≥ 10 nM, CRF and two analogous peptides (Tyr-CRF and α-helical CRF) elicited 16- to 21-fold stimulation of IL-6 production by MNC. Purified monocytes, but not purified lymphocytes, were the cells that responded to CRF action exhibiting nearly 19-fold stimulation at 100 nM concentration. The CRF-induced production of IL-6 cytokine by peripheral blood MNC may suggest a messenger role for this neurohormone in the feedback control of neuroendocrine-immune circuitry.
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U2 - 10.1016/0008-8749(92)90018-K
DO - 10.1016/0008-8749(92)90018-K
M3 - Article
C2 - 1623564
AN - SCOPUS:0026680727
SN - 0008-8749
VL - 143
SP - 220
EP - 227
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -