TY - JOUR
T1 - Static magnetic field increases survival rate of dental pulp stem cells during DMSO-free cryopreservation
AU - Lin, Shu Li
AU - Chang, Wei-Jen
AU - Lin, Chun Yen
AU - Hsien, Sung-Chih
AU - Lee, Sheng-Yang
AU - Fan, Kang Hsin
AU - Lin, Che-Tong
AU - Huang, Haw-Ming
N1 - Publisher Copyright:
© 2014 © 2014 Taylor & Francis.
PY - 2015
Y1 - 2015
N2 - Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective effects of static magnetic fields (SMFs) on human dental pulp stem cells (DPSCs) during cryopreservation. Human DPSCs isolated from extracted teeth were frozen with a 0.4-T or 0.8-T SMF and then stored at −196 °C for 24 h. During freezing, the cells were suspended in freezing media containing with 0, 3 or 10% DMSO. After thawing, the changes in survival rate of the DPSCs were determined by flow cytometry. To understand the possible cryoprotective mechanisms of the SMF, the membrane fluidity of SMF-exposed DPSCs was tested. The results showed that when the freezing medium was DMSO-free, the survival rates of the thawed DPSCs increased 2- or 2.5-fold when the cells were exposed to 0.4-T or 0.8-T SMFs, respectively (p
AB - Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective effects of static magnetic fields (SMFs) on human dental pulp stem cells (DPSCs) during cryopreservation. Human DPSCs isolated from extracted teeth were frozen with a 0.4-T or 0.8-T SMF and then stored at −196 °C for 24 h. During freezing, the cells were suspended in freezing media containing with 0, 3 or 10% DMSO. After thawing, the changes in survival rate of the DPSCs were determined by flow cytometry. To understand the possible cryoprotective mechanisms of the SMF, the membrane fluidity of SMF-exposed DPSCs was tested. The results showed that when the freezing medium was DMSO-free, the survival rates of the thawed DPSCs increased 2- or 2.5-fold when the cells were exposed to 0.4-T or 0.8-T SMFs, respectively (p
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-84947039986&origin=resultslist&sort=plf-f&src=s&st1=Static+magnetic+field+increases+survival+rate+of+dental+pulp+stem+cells+during+DMSO-free+cryopreservation&st2=&sid=159A47CFA43817B73494F7C12B4A65CD.wsnAw8kcdt7IPYLO0V48gA%3a1590&sot=b&sdt=b&sl=120&s=TITLE-ABS-KEY%28Static+magnetic+field+increases+survival+rate+of+dental+pulp+stem+cells+during+DMSO-free+cryopreservation%29&relpos=0&citeCnt=4&searchTerm=
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U2 - 10.3109/15368378.2014.919588
DO - 10.3109/15368378.2014.919588
M3 - Article
C2 - 24856869
AN - SCOPUS:84947039986
SN - 1536-8378
VL - 34
SP - 302
EP - 308
JO - Electromagnetic Biology and Medicine
JF - Electromagnetic Biology and Medicine
IS - 4
ER -
