Sperm mitochondrial DNA depletion in men with asthenospermia

Shu Huei Kao, Hsiang Tai Chao, Hwan Wun Liu, Tien Lin Liao, Yau Huei Wei

研究成果: 雜誌貢獻文章同行評審

67 引文 斯高帕斯(Scopus)


Objective To determine the content of sperm mitochondrial DNA (mtDNA) in patients with asthenospermia or with poor sperm motility. Design Analysis of the content of mtDNA as the ratio between the amount of mtDNA and nuclear DNA by using a new concurrent polymerase chain reaction. Setting University hospital infertility center. Patient(s) Eighty-six men who sought infertility therapy. Intervention(s) Moving characteristics of sperm were examined with a computer-assisted semen analyzer. Main outcome measure(s) Sperm samples were classified into two groups, one with asthenospermia and the other with normal moving characteristics. The content of mtDNA in sperm was determined by polymerase chain reaction. We analyzed the mitochondrial mass by MitoTracker Green staining and analyzed DNA content with propidium iodide staining by flow cytometry. Result(s) A decrease in sperm mtDNA content was detected in patients with asthenospermia or with poor sperm motility (
頁(從 - 到)66-73
期刊Fertility and Sterility
出版狀態已發佈 - 7月 2004

ASJC Scopus subject areas

  • 婦產科
  • 生殖醫學


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