TY - JOUR
T1 - Soot-driven reactive oxygen species formation from incense burning
AU - Chuang, Hsiao Chi
AU - Jones, Tim P.
AU - Lung, Shih Chun C
AU - BéruBé, Kelly A.
PY - 2011/10/15
Y1 - 2011/10/15
N2 - This study investigated the effects of reactive oxygen species (ROS) generated as a function of the physicochemistry of incense particulate matter (IPM), diesel exhaust particles (DEP) and carbon black (CB). Microscopical and elemental analyses were used to determine particle morphology and inorganic compounds. ROS was determined using the reactive dye, Dichlorodihydrofluorescin (DCFH), and the Plasmid Scission Assay (PSA), which determine DNA damage. Two common types of soot were observed within IPM, including nano-soot and micro-soot, whereas DEP and CB mainly consisted of nano-soot. These PM were capable of causing oxidative stress in a dose-dependent manner, especially IPM and DEP. A dose of IPM (36.6-102.3. μg/ml) was capable of causing 50% oxidative DNA damage. ROS formation was positively correlated to smaller nano-soot aggregates and bulk metallic compounds, particularly Cu. These observations have important implications for respiratory health given that inflammation has been recognised as an important factor in the development of lung injury/diseases by oxidative stress. This study supports the view that ROS formation by combustion-derived PM is related to PM physicochemistry, and also provides new data for IPM.
AB - This study investigated the effects of reactive oxygen species (ROS) generated as a function of the physicochemistry of incense particulate matter (IPM), diesel exhaust particles (DEP) and carbon black (CB). Microscopical and elemental analyses were used to determine particle morphology and inorganic compounds. ROS was determined using the reactive dye, Dichlorodihydrofluorescin (DCFH), and the Plasmid Scission Assay (PSA), which determine DNA damage. Two common types of soot were observed within IPM, including nano-soot and micro-soot, whereas DEP and CB mainly consisted of nano-soot. These PM were capable of causing oxidative stress in a dose-dependent manner, especially IPM and DEP. A dose of IPM (36.6-102.3. μg/ml) was capable of causing 50% oxidative DNA damage. ROS formation was positively correlated to smaller nano-soot aggregates and bulk metallic compounds, particularly Cu. These observations have important implications for respiratory health given that inflammation has been recognised as an important factor in the development of lung injury/diseases by oxidative stress. This study supports the view that ROS formation by combustion-derived PM is related to PM physicochemistry, and also provides new data for IPM.
KW - CB
KW - DEP
KW - Incense
KW - Physicochemistry
KW - ROS
KW - Soot
UR - http://www.scopus.com/inward/record.url?scp=80053298983&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053298983&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2011.07.041
DO - 10.1016/j.scitotenv.2011.07.041
M3 - Article
C2 - 21889784
AN - SCOPUS:80053298983
SN - 0048-9697
VL - 409
SP - 4781
EP - 4787
JO - Science of the Total Environment
JF - Science of the Total Environment
IS - 22
ER -