Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells

Chung Ren Lin, Kuan Hung Chen, Chien Hui Yang, Jiin Tsuey Cheng, Shyr Ming Sheen-Chen, Chih Hsien Wu, Wei Dih Sy, Yi Shen Chen

研究成果: 雜誌貢獻文章同行評審

18 引文 斯高帕斯(Scopus)


Background: Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods: Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results: The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. Conclusions: Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons.

期刊Journal of Biomedical Science
出版狀態已發佈 - 1月 1 2010

ASJC Scopus subject areas

  • 內分泌學、糖尿病和代謝
  • 分子生物學
  • 臨床生物化學
  • 細胞生物學
  • 生物化學(醫學)
  • 藥學(醫學)


深入研究「Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells」主題。共同形成了獨特的指紋。