TY - JOUR
T1 - Sintered β-dicalcium phosphate particles induce intracellular reactive oxygen species in rat osteoblasts
AU - Liu, H. C.
AU - Chen, R. M.
AU - Lin, F. H.
AU - Fang, H. W.
PY - 1999/10/25
Y1 - 1999/10/25
N2 - Osteolysis was usually associated with the wear particles from biomaterials used in the artificial joint, but the mechanism is not well known. This study was aimed to investigate the effects of sintered β-dicalcium phosphate (SDCP) particles on induction of intracellular reactive oxygen species and on inhibition of cell viability and alkaline phosphatase activity in rat calvarial osteoblasts. Analysis of cell viability revealed that smaller particles of SDCP caused more cell death, and the effect was time-dependent. Treatment of the osteoblastic cells with SDCP particles of various sizes resulted in decrease of alkaline phosphatase activity, and the suppressive effect was dependent on particle sizes and treating time intervals. SDCP particles induced the production of intracellular reactive oxygen species in rat osteoblasts. The wear particles of SDCP with smaller sizes induced more intracellular reactive oxygen species of osteoblasts. The present study is the first report to identify that the particles of SDCP could induce oxidative stress in osteoblastic cells. From these data we propose that intracellular reactive oxygen species induced by SDCP particles may be involved in SDCP particles-caused cell death and decrease of alkaline phosphatase activity in rat osteoblasts.
AB - Osteolysis was usually associated with the wear particles from biomaterials used in the artificial joint, but the mechanism is not well known. This study was aimed to investigate the effects of sintered β-dicalcium phosphate (SDCP) particles on induction of intracellular reactive oxygen species and on inhibition of cell viability and alkaline phosphatase activity in rat calvarial osteoblasts. Analysis of cell viability revealed that smaller particles of SDCP caused more cell death, and the effect was time-dependent. Treatment of the osteoblastic cells with SDCP particles of various sizes resulted in decrease of alkaline phosphatase activity, and the suppressive effect was dependent on particle sizes and treating time intervals. SDCP particles induced the production of intracellular reactive oxygen species in rat osteoblasts. The wear particles of SDCP with smaller sizes induced more intracellular reactive oxygen species of osteoblasts. The present study is the first report to identify that the particles of SDCP could induce oxidative stress in osteoblastic cells. From these data we propose that intracellular reactive oxygen species induced by SDCP particles may be involved in SDCP particles-caused cell death and decrease of alkaline phosphatase activity in rat osteoblasts.
KW - Alkaline phosphatase
KW - Cell viability
KW - Intracellular reactive oxygen species
KW - Osteoblast
KW - Particle size
KW - Sintered β-dicalcium phosphate
UR - https://www.scopus.com/pages/publications/0033604150
UR - https://www.scopus.com/pages/publications/0033604150#tab=citedBy
M3 - Article
AN - SCOPUS:0033604150
SN - 1016-2372
VL - 11
SP - 259
EP - 264
JO - Biomedical Engineering - Applications, Basis and Communications
JF - Biomedical Engineering - Applications, Basis and Communications
IS - 5
ER -