ShyA, a membrane protein for proper septation of hyphae in Streptomyces

Shun-Fu Tseng, Tzu-Wen Huang, Hsiuh-Wei Chen, Ming-Kai Chern, Ming-F. Tam, Shu-Chun Teng

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)


The life cycle of Streptomyces involves the formation of filamentous substrate and aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to produce a chain of unigenomic spores. A gene, shyA, which influences several aspects of this growth, was isolated and partially characterized in Streptomyces coelicolor. The gene product is a representative of a well-conserved family of small actinomycete proteins. The shyA mutant sporulates normally but displays hyper septum formation and altered spore-chain morphology. Biochemical separation experiments and immunofluorescence staining demonstrated that the shyA gene product locates at cell membranes. Moreover, yeast two-hybrid screen and GST-pull-down assay showed that ShyA can interact with itself. Altogether, ShyA belongs to a new family of membrane-associated proteins which plays a role in morphological differentiation in actinomycetes. © 2006 Elsevier Inc. All rights reserved.
頁(從 - 到)369-377
期刊Biochemical and Biophysical Research Communications
出版狀態已發佈 - 2006


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