TY - JOUR
T1 - Sequential up-regulation of the c-fos, c-jun and bax genes in the cortex, striatum and cerebellum induced by a single injection of a low dose of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in C57BL/6 mice
AU - Chen, Yi-Ru
AU - Hsu, Ping Ching
AU - Hsu, Iee Lin
AU - Yeh, Geng Chang
PY - 2001/11/13
Y1 - 2001/11/13
N2 - We investigated whether single injection of 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) (20 mg/kg) will alter the expression of pro-apoptotic genes, namely, the c-fos, c-jun, and bax, in the striatum, cortex, and cerebellum of adult male C57BL/6 mice using reverse transcription-polymerase chain reaction assay. Injection of MPTP induced a transient decrease in the content of tyrosine hydroxylase estimated by the immunoreactivity in the striatum, which completely recovered 14 day after injection. A rapid but transient up-regulation of c-fos and c-jun genes occurred an hour after MPTP-injection, and a delayed but persistent up-regulation of bax gene expression occurred 3 day after injection. The up-regulation of these genes was present in all the examined brain regions. This result suggests that MPTP, at a low dose causing transient degeneration in the striatum, is capable of triggering two genetic pathways related to the generation of apoptosis in both dopaminergic and non-dopaminergic systems in the mouse brain.
AB - We investigated whether single injection of 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) (20 mg/kg) will alter the expression of pro-apoptotic genes, namely, the c-fos, c-jun, and bax, in the striatum, cortex, and cerebellum of adult male C57BL/6 mice using reverse transcription-polymerase chain reaction assay. Injection of MPTP induced a transient decrease in the content of tyrosine hydroxylase estimated by the immunoreactivity in the striatum, which completely recovered 14 day after injection. A rapid but transient up-regulation of c-fos and c-jun genes occurred an hour after MPTP-injection, and a delayed but persistent up-regulation of bax gene expression occurred 3 day after injection. The up-regulation of these genes was present in all the examined brain regions. This result suggests that MPTP, at a low dose causing transient degeneration in the striatum, is capable of triggering two genetic pathways related to the generation of apoptosis in both dopaminergic and non-dopaminergic systems in the mouse brain.
KW - Apoptosis
KW - Bax
KW - C-fos
KW - C-jun
KW - MPTP
KW - Mouse brain
KW - Reverse transcription-polymerase chain reaction
KW - Tyrosine hydroxylase
UR - http://www.scopus.com/inward/record.url?scp=0035856398&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035856398&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(01)02281-9
DO - 10.1016/S0304-3940(01)02281-9
M3 - Article
C2 - 11698144
AN - SCOPUS:0035856398
SN - 0304-3940
VL - 314
SP - 49
EP - 52
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -