@article{a8bbb905146a472aa2daebf65a5a5a7f,
title = "Safe and easy emergence from anesthesia in adults following removal of laryngeal mask airway: Utility of oral airway and T-connector",
abstract = "Removal of the laryngeal mask airway (LMA) can be executed while patients are deeply anesthetized or awake. Recent publications have focused on suitable anesthetic concentrations in the brain for removal of LMA in anesthetized patients. Here, we describe an easy and safe method for removal of LMA during deep anesthesia.",
keywords = "Anesthesia recovery period, emergence, Laryngeal masks",
author = "Hsieh, {Ming H.} and Ho, {Jin T.} and Huang, {Chin M.} and Lee, {Meei Shyuan} and Ta-Liang Chen and Wong, {Chih Shung} and Jui-An Lin",
note = "Funding Information: This work was supported by a Grant from Taipei Medical University and Wan Fang Hospital ( 102TMU-WFH-03 ) and was partly supported by Grants from the National Science Council ( NSC101-2320-B-038-006 and NSC102-2320-B-038-020-MY2 ), Taiwan. Funding Information: We obtained 2 human RCC cell lines A498 and 786-O from the American Type Culture Collection and routinely cultured them in minimum essential medium or Roswell Park Memorial Institute medium, supplemented with 10% fetal bovine serum (FBS) and 1% MycoZap Plus-CL antibiotics (Lonza, Verviers, Belgium). Cells were cultured at 37°C in a humidified incubator containing 5% CO 2 . The constructs with scrambled and 2 Anxa2 short hairpin (sh)RNA reagents were obtained from the National RNAi Core Facility Platform located at the Institute of Molecular Biology/Genomic Research Center, Academia Sinica (Taipei, Taiwan), supported by the National Core Facility Program for Biotechnology Grants of the National Science Council (NSC100-2319-B-001-002). The sequence of the Anxa2-KD1-shRNA oligonucleotide was 5′-CCGGGCAGGAAATTAACAGAGTCTACTCGAGTAGACTCTGTTAATTTCCTGCTTTTTG-3′ and of the Anxa2-KD2-shRNA oligonucleotide was 5′-CCGGCGGGATGCTTTGAACATTGAACTCGAGTTCAATGTTCAAAGCATCCCGTTTTTG-3′. The transfected cells were selected using 1 μg/ml of puromycin (Invitrogen, San Diego, CA), and the knockdown efficiency was confirmed by real-time polymerase chain reaction (PCR) and Western blot analysis. The Rock inhibitor Y-27632 and human Cdc42 small interfering (si)RNA (sc-29256, Santa Cruz, Santa Cruz, CA) were applied for the wound-healing and invasion assay. ",
year = "2009",
month = jun,
doi = "10.1016/S1875-4597(09)60029-1",
language = "English",
volume = "47",
pages = "84--86",
journal = "Acta Anaesthesiologica Taiwanica",
issn = "1875-4597",
publisher = "Elsevier Taiwan LLC",
number = "2",
}