Role of pirenoxine in the effects of catalin on in vitro ultravioletinduced lens protein turbidity and selenite-induced cataractogenesis in vivo

Chao Chien Hu; Jiahn Haur Liao; Kuang Yang Hsu; I. Lin Lin; Ming Hsuan Tsai; Wen Hsin Wu; Tzu Tang Wei; Yi Shiang Huang; Shih Jiuan Chiu; Hsiang Yin Chen; Shih Hsiung Wu; Tzu Hua Wu

Role of pirenoxine in the effects of catalin on in vitro ultravioletinduced lens protein turbidity and selenite-induced cataractogenesis in vivo

Chao Chien Hu, Jiahn Haur Liao, Kuang Yang Hsu, I. Lin Lin, Ming Hsuan Tsai, Wen Hsin Wu, Tzu Tang Wei, Yi Shiang Huang, Shih Jiuan Chiu, Hsiang Yin Chen, Shih Hsiung Wu, Tzu Hua Wu

研究成果: 雜誌貢獻 › 文章 › 同行評審

22 引文斯高帕斯（Scopus）

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Purpose: In this study, we investigated the biochemical pharmacology of pirenoxine (PRX) and catalin under in vitro selenite/calcium- and ultraviolet (UV)-induced lens protein turbidity challenges. The systemic effects of catalin were determined using a selenite-induced cataractogenesis rat model. Methods: In vitro cataractogenesis assay systems (including UVB/C photo-oxidation of lens crystallins, calpain-induced proteolysis, and selenite/calcium-induced turbidity of lens crystallin solutions) were used to screen the activity of PRX and catalin eye drop solutions. Turbidity was identified as the optical density measured using spectroscopy at 405 nm. We also determined the in vivo effects of catalin on cataract severity in a selenite-induced cataract rat model. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze the integrity of crystallin samples. Results: PRX at 1,000 μM significantly delayed UVC-induced turbidity formation compared to controls after 4 h of UVC exposure (p

原文	英語
頁（從 - 到）	1862-1870
頁數	9
期刊	Molecular Vision
卷	17
出版狀態	已發佈 - 2011

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title = "Role of pirenoxine in the effects of catalin on in vitro ultravioletinduced lens protein turbidity and selenite-induced cataractogenesis in vivo",

abstract = "Purpose: In this study, we investigated the biochemical pharmacology of pirenoxine (PRX) and catalin under in vitro selenite/calcium- and ultraviolet (UV)-induced lens protein turbidity challenges. The systemic effects of catalin were determined using a selenite-induced cataractogenesis rat model. Methods: In vitro cataractogenesis assay systems (including UVB/C photo-oxidation of lens crystallins, calpain-induced proteolysis, and selenite/calcium-induced turbidity of lens crystallin solutions) were used to screen the activity of PRX and catalin eye drop solutions. Turbidity was identified as the optical density measured using spectroscopy at 405 nm. We also determined the in vivo effects of catalin on cataract severity in a selenite-induced cataract rat model. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze the integrity of crystallin samples. Results: PRX at 1,000 μM significantly delayed UVC-induced turbidity formation compared to controls after 4 h of UVC exposure (p",

author = "Hu, {Chao Chien} and Liao, {Jiahn Haur} and Hsu, {Kuang Yang} and Lin, {I. Lin} and Tsai, {Ming Hsuan} and Wu, {Wen Hsin} and Wei, {Tzu Tang} and Huang, {Yi Shiang} and Chiu, {Shih Jiuan} and Chen, {Hsiang Yin} and Wu, {Shih Hsiung} and Wu, {Tzu Hua}",

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T1 - Role of pirenoxine in the effects of catalin on in vitro ultravioletinduced lens protein turbidity and selenite-induced cataractogenesis in vivo

AU - Hu, Chao Chien

AU - Liao, Jiahn Haur

AU - Hsu, Kuang Yang

AU - Lin, I. Lin

AU - Tsai, Ming Hsuan

AU - Wu, Wen Hsin

AU - Wei, Tzu Tang

AU - Huang, Yi Shiang

AU - Chiu, Shih Jiuan

AU - Chen, Hsiang Yin

AU - Wu, Shih Hsiung

AU - Wu, Tzu Hua

PY - 2011

Y1 - 2011

N2 - Purpose: In this study, we investigated the biochemical pharmacology of pirenoxine (PRX) and catalin under in vitro selenite/calcium- and ultraviolet (UV)-induced lens protein turbidity challenges. The systemic effects of catalin were determined using a selenite-induced cataractogenesis rat model. Methods: In vitro cataractogenesis assay systems (including UVB/C photo-oxidation of lens crystallins, calpain-induced proteolysis, and selenite/calcium-induced turbidity of lens crystallin solutions) were used to screen the activity of PRX and catalin eye drop solutions. Turbidity was identified as the optical density measured using spectroscopy at 405 nm. We also determined the in vivo effects of catalin on cataract severity in a selenite-induced cataract rat model. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze the integrity of crystallin samples. Results: PRX at 1,000 μM significantly delayed UVC-induced turbidity formation compared to controls after 4 h of UVC exposure (p

AB - Purpose: In this study, we investigated the biochemical pharmacology of pirenoxine (PRX) and catalin under in vitro selenite/calcium- and ultraviolet (UV)-induced lens protein turbidity challenges. The systemic effects of catalin were determined using a selenite-induced cataractogenesis rat model. Methods: In vitro cataractogenesis assay systems (including UVB/C photo-oxidation of lens crystallins, calpain-induced proteolysis, and selenite/calcium-induced turbidity of lens crystallin solutions) were used to screen the activity of PRX and catalin eye drop solutions. Turbidity was identified as the optical density measured using spectroscopy at 405 nm. We also determined the in vivo effects of catalin on cataract severity in a selenite-induced cataract rat model. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze the integrity of crystallin samples. Results: PRX at 1,000 μM significantly delayed UVC-induced turbidity formation compared to controls after 4 h of UVC exposure (p

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AN - SCOPUS:79960713149

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Role of pirenoxine in the effects of catalin on in vitro ultravioletinduced lens protein turbidity and selenite-induced cataractogenesis in vivo

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