TY - JOUR
T1 - Role of oxygen-derived free radicals in the mechanism of cysteamine-induced duodenal ulcer in rats
AU - Chen, S. H.
AU - Pan, S.
AU - Okita, K.
AU - Takemoto, T.
PY - 1994
Y1 - 1994
N2 - In this study, the possible role of oxygen-derived free radicals in cysteamine-induced duodenal ulcers in rats was investigated. Separate groups of rats were treated with 350 mg/kg cysteamine subcutaneously. Superoxide dismutase (SOD) activity and thiobarbituric acid (TBA) reactants in the duodenal mucosa were analyzed one, three, six, 12 and 24 hours after treatment, respectively. Cu,Zn-SOD activity significantly decreased from 41.79 ± 3.53 NU/mg protein to 29.56 ± 3.62, 27.23 ± 3.02 and 24.64 ± 3.07 NU/mg protein at three, six and 12 hours, respectively. TBA reactants, indicators of tissue injury by oxygen-derived free radicals, significantly increased from 0.18 ± 0.10 nmol/mg protein to 1.31 ± 0.12 and 1.57 ± 0.09 nmol/mg protein at six and 12 hours after cysteamine treatment, respectively. We conclude that an increase of oxygen-derived free radicals and a decrease of Cu,Zn-SOD activity in the duodenal mucosa may be involved in the pathogenesis of cysteamine-induced duodenal ulcers in rats.
AB - In this study, the possible role of oxygen-derived free radicals in cysteamine-induced duodenal ulcers in rats was investigated. Separate groups of rats were treated with 350 mg/kg cysteamine subcutaneously. Superoxide dismutase (SOD) activity and thiobarbituric acid (TBA) reactants in the duodenal mucosa were analyzed one, three, six, 12 and 24 hours after treatment, respectively. Cu,Zn-SOD activity significantly decreased from 41.79 ± 3.53 NU/mg protein to 29.56 ± 3.62, 27.23 ± 3.02 and 24.64 ± 3.07 NU/mg protein at three, six and 12 hours, respectively. TBA reactants, indicators of tissue injury by oxygen-derived free radicals, significantly increased from 0.18 ± 0.10 nmol/mg protein to 1.31 ± 0.12 and 1.57 ± 0.09 nmol/mg protein at six and 12 hours after cysteamine treatment, respectively. We conclude that an increase of oxygen-derived free radicals and a decrease of Cu,Zn-SOD activity in the duodenal mucosa may be involved in the pathogenesis of cysteamine-induced duodenal ulcers in rats.
KW - cysteamine
KW - duodenal ulcer
KW - lipid peroxidation
KW - oxygen-derived free radicals
KW - superoxide dismutase
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M3 - Article
C2 - 7915573
AN - SCOPUS:0028276228
SN - 0929-6646
VL - 93
SP - 11
EP - 14
JO - Journal of the Formosan Medical Association
JF - Journal of the Formosan Medical Association
IS - 1
ER -