Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture

Ying Ju Lin; Chia Yen Chen; Kuan Teh Jeang; Xiang Liu; Jen Hsien Wang; Chien Hui Hung; Hsinyi Tsang; Ting Hsu Lin; Chiu Chu Liao; Shao Mei Huang; Cheng Wen Lin; Mao Wang Ho; Wen Kuei Chien; Jin Hua Chen; Tsung Jung Ho; Fuu Jen Tsai

doi:10.1186/2045-3701-4-40

Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture

Ying Ju Lin, Chia Yen Chen, Kuan Teh Jeang, Xiang Liu, Jen Hsien Wang, Chien Hui Hung, Hsinyi Tsang, Ting Hsu Lin, Chiu Chu Liao, Shao Mei Huang, Cheng Wen Lin, Mao Wang Ho, Wen Kuei Chien, Jin Hua Chen, Tsung Jung Ho, Fuu Jen Tsai

研究成果: 雜誌貢獻 › 文章 › 同行評審

5 引文斯高帕斯（Scopus）

摘要

Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.

原文	英語
文章編號	40
期刊	Cell and Bioscience
卷	4
發行號	1
DOIs	https://doi.org/10.1186/2045-3701-4-40
出版狀態	已發佈 - 8月 5 2014

ASJC Scopus subject areas

一般生物化學，遺傳學和分子生物學

UN SDG

此研究成果有助於以下永續發展目標

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10.1186/2045-3701-4-40

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Lin, Y. J., Chen, C. Y., Jeang, K. T., Liu, X., Wang, J. H., Hung, C. H., Tsang, H., Lin, T. H., Liao, C. C., Huang, S. M., Lin, C. W., Ho, M. W., Chien, W. K., Chen, J. H., Ho, T. J., & Tsai, F. J. (2014). Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture. Cell and Bioscience, 4(1), 文章 40. https://doi.org/10.1186/2045-3701-4-40

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title = "Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture",

abstract = "Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.",

keywords = "HIV-1 viral load, RNF39, Single nucleotide polymorphism, Viral replication",

author = "Lin, {Ying Ju} and Chen, {Chia Yen} and Jeang, {Kuan Teh} and Xiang Liu and Wang, {Jen Hsien} and Hung, {Chien Hui} and Hsinyi Tsang and Lin, {Ting Hsu} and Liao, {Chiu Chu} and Huang, {Shao Mei} and Lin, {Cheng Wen} and Ho, {Mao Wang} and Chien, {Wen Kuei} and Chen, {Jin Hua} and Ho, {Tsung Jung} and Tsai, {Fuu Jen}",

note = "Publisher Copyright: {\textcopyright} 2014 Lin et al.; licensee BioMed Central Ltd.",

year = "2014",

month = aug,

day = "5",

doi = "10.1186/2045-3701-4-40",

language = "English",

volume = "4",

journal = "Cell and Bioscience",

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TY - JOUR

T1 - Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture

AU - Lin, Ying Ju

AU - Chen, Chia Yen

AU - Jeang, Kuan Teh

AU - Liu, Xiang

AU - Wang, Jen Hsien

AU - Hung, Chien Hui

AU - Tsang, Hsinyi

AU - Lin, Ting Hsu

AU - Liao, Chiu Chu

AU - Huang, Shao Mei

AU - Lin, Cheng Wen

AU - Ho, Mao Wang

AU - Chien, Wen Kuei

AU - Chen, Jin Hua

AU - Ho, Tsung Jung

AU - Tsai, Fuu Jen

PY - 2014/8/5

Y1 - 2014/8/5

N2 - Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.

AB - Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.

KW - HIV-1 viral load

KW - RNF39

KW - Single nucleotide polymorphism

KW - Viral replication

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Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture

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