Requirement of MMP-3 in anchorage-independent growth of oral squamous cell carcinomas

Shyun Yeu Liu, Young Chau Liu, Wen Tsung Huang, Guan Cheng Huang, Hsiao Jing Su, Mei Huei Lin

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)


Background: Matrix metalloproteinase-3 (MMP-3) is expressed in various carcinomas; however, its function is not clearly established. This study was to assess its possible role in oral squamous cell carcinomas (OSCCs). Materials and methods: Specimens of seven oral pre-malignant lesions (OPMLs) and 92 OSCCs were subjected to MMP-3 detection by RT-PCR and Western blot. Antisense oligodeoxynucleotides (AODNs) of MMP-3 were used to transfect OSCC (OECM-1 and SCC-9) and esophageal carcinoma (CE81T/VGH) cell lines, and their growth was subsequently analyzed by XTT and soft-agar colony assay. Results: MMP-3 transcript was preferentially expressed in OSCCs (71 of 92, 77%) than in OPMLs (two of seven, 29%; P = 0.012). Both MMP-3 transcript and protein levels were significantly higher in OSCC masses than in neighboring tissues (P <0.0001 and P = 0.04, respectively). Growth of the three cell lines was not affected, while the colony numbers of OECM-1 and CE81T/VGH were significantly reduced by the transfection of MMP-3 AODNs (P = 0.002 and P = 0.004, respectively). SCC-9 did not form colonies in soft-agar/medium. Conclusions: MMP-3 function may be required in most OSCCs, and it may support the anchorage-independent growth of both OSCC and esophageal carcinoma.
頁(從 - 到)430-435
期刊Journal of Oral Pathology and Medicine
出版狀態已發佈 - 8月 2007

ASJC Scopus subject areas

  • 癌症研究
  • 病理學與法醫學
  • 牙科 (全部)


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