Repeated ovarian stimulations induce oxidative damage and mitochondrial DNA mutations in mouse ovaries

Hsiang Tai Chao, Shu Yu Lee, Horng Mo Lee, Tien Ling Liao, Yau Huei Wei, Shu Huei Kao

研究成果: 雜誌貢獻文章同行評審

76 引文 斯高帕斯(Scopus)


Superovulation by injection of exogenous gonadotropin is the elementary method to produce in vivo-derived embryos for embryo transfer in women. Increased oocyte aneuploidy, embryo mortality, fetal growth retardation, and congenital abnormalities have been studied at higher-dose stimulations. Ovarian and oocyte biological aging possibly may have adverse implications for human oocyte competence with repeated hyperstimulation. In this study, we found that reduced competence for the human oocyte has been associated with degenerative embryo upsurge during embryo culture and failure to develop into the blastocyst stage in the three, four, five, and six stimulation cycles. On the other hand, the numbers of ovulated oocytes were decreased in the groups with more ovarian stimulation. More aggregated mitochondria were found in the cytoplasm of the repetitively stimulated embryos. Higher amounts of oxidative damage including 8-OH-dG, lipoperoxides, and carbonyl proteins were also revealed in the ovaries with more cycle numbers of ovarian stimulation. Higher proportions of mtDNA mutations were also found. The detected molecular size of the mutated band was approximately 675 bp. Increased amounts of carbonyl proteins were also revealed after repeated stimulation. An understanding of the relationship between oocyte competence and ovarian responses to stimulation in the mouse may provide insights into the origin of oocyte defects and the biology of ooplasmic aging that could be of clinical relevance in the diagnosis and treatment of human infertility.
頁(從 - 到)148-156
期刊Annals of the New York Academy of Sciences
出版狀態已發佈 - 2005

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)
  • 科學史與哲學


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