摘要
Bone formation steadily declines with age resulting in a loss of bone mass. Reactive oxygen species (ROS) are thought to be major contributors to the aging process. The zinc finger transcription factor, Early Growth Response-1 (Egr-1) is a potential regulator as a transcription factor of many target genes, and plays a role in cell growth, development, and differentiation. However, the effect of ageing on Egr-1 gene expression has not yet been ascertained. To identify which genes have an altered transcription level associated with bone loss by ageing, the pre-osteoblastic cell MC3T3-E1 was treated with H 2O2, and gene expression profiles analyzed with gene chip technology using the Affymetrix GeneChip analysis system (Mouse; 34,000 genes). The expression of many genes in MC3T3-E1 was altered significantly with Egr-1 being decreased by H2O2. The reduction of Egr-1 mRNA levels was successfully confirmed by reversed transcription polymerase chain reaction (RT-PCR) and real-time PCR. Since it has been reported that Egr-1 plays an important role as a transcription factor for growth factor genes which promote cell proliferation and differentiation of preosteoblastic cells, the reduction of Egr-1 gene expression by H2O2 may be involved in the decline of bone formation in the ageing process.
原文 | 英語 |
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頁(從 - 到) | 31-34 |
頁數 | 4 |
期刊 | Journal of Hard Tissue Biology |
卷 | 17 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 2008 |
ASJC Scopus subject areas
- 醫藥(雜項)
- 生物化學
- 生物材料
- 骨科和運動醫學
- 一般牙醫學
- 細胞生物學