TY - JOUR
T1 - Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene
AU - Chen, Yi Chung
AU - Cheng, Chia Hsiung
AU - Chen, Gen Der
AU - Hung, Chin Chun
AU - Yang, Chung Hsiang
AU - Hwang, Sheng Ping L
AU - Kawakami, Koichi
AU - Wu, Bo Kai
AU - Huang, Chang Jen
PY - 2009/3
Y1 - 2009/3
N2 - Human synuclein family consists of α-, β-, and γ-synucleins. Here, we cloned three genes, sncb, sncga and sncgb from zebrafish. They encode β-, γ1-, and γ2-synucleins, respectively. The zSyn-γ, zSyn-γ1, and zSyn-γ2 proteins display 69%, 47%, and 50% identity to human β-synuclein and γ-synuclein, respectively. By reverse transcriptase-polymerase chain reaction, we demonstrated that sncb and sncga mRNA were abundant in brain and eye, while sncgb expression was moderate in brain, kidney, ovary and testis. The 1.8-kb 5′-upstream/promoter region of the sncga gene was sufficient to direct green fluorescent protein (GFP) expression in the central nervous system and cranial ganglions. A transgenic line, Tg(sncga:GFP), was generated and its GFP expression is similar to that of endogenous sncga mRNA. Moreover, this line also labels the habenular complex and the domain of GFP expression is larger in the left than in the right habenula. Thus, this line can be used to study sncga gene regulation and for left-right asymmetry study in zebrafish brain.
AB - Human synuclein family consists of α-, β-, and γ-synucleins. Here, we cloned three genes, sncb, sncga and sncgb from zebrafish. They encode β-, γ1-, and γ2-synucleins, respectively. The zSyn-γ, zSyn-γ1, and zSyn-γ2 proteins display 69%, 47%, and 50% identity to human β-synuclein and γ-synuclein, respectively. By reverse transcriptase-polymerase chain reaction, we demonstrated that sncb and sncga mRNA were abundant in brain and eye, while sncgb expression was moderate in brain, kidney, ovary and testis. The 1.8-kb 5′-upstream/promoter region of the sncga gene was sufficient to direct green fluorescent protein (GFP) expression in the central nervous system and cranial ganglions. A transgenic line, Tg(sncga:GFP), was generated and its GFP expression is similar to that of endogenous sncga mRNA. Moreover, this line also labels the habenular complex and the domain of GFP expression is larger in the left than in the right habenula. Thus, this line can be used to study sncga gene regulation and for left-right asymmetry study in zebrafish brain.
KW - Habenular nuclei
KW - Interpeduncular nucleus
KW - Promoter
KW - Synuclein
KW - Tol2-mediated transgenesis
KW - Zebrafish
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UR - http://www.scopus.com/inward/citedby.url?scp=61649087654&partnerID=8YFLogxK
U2 - 10.1002/dvdy.21877
DO - 10.1002/dvdy.21877
M3 - Article
C2 - 19235732
AN - SCOPUS:61649087654
SN - 1058-8388
VL - 238
SP - 746
EP - 754
JO - Developmental Dynamics
JF - Developmental Dynamics
IS - 3
ER -