Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells

Hsin Yi Chang, Ming Hua Li, Tsui Chin Huang, Chia Lang Hsu, Shang Ru Tsai, Si Chen Lee, Hsuan Cheng Huang, Hsueh Fen Juan

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)


Breast cancer is one of the leading cancer-related causes of death worldwide. Treatment of triple-negative breast cancer (TNBC) is complex and challenging, especially when metastasis has developed. In this study, we applied infrared radiation as an alternative approach for the treatment of TNBC. We used middle infrared (MIR) with a wavelength range of 3-5 Îm to irradiate breast cancer cells. MIR significantly inhibited cell proliferation in several breast cancer cells but did not affect the growth of normal breast epithelial cells. We performed iTRAQ-coupled LC-MS/MS analysis to investigate the MIR-triggered molecular mechanisms in breast cancer cells. A total of 1749 proteins were identified, quantified, and subjected to functional enrichment analysis. From the constructed functionally enriched network, we confirmed that MIR caused G2/M cell cycle arrest, remodeled the microtubule network to an astral pole arrangement, altered the actin filament formation and focal adhesion molecule localization, and reduced cell migration activity and invasion ability. Our results reveal the coordinative effects of MIR-regulated physiological responses in concentrated networks, demonstrating the potential implementation of infrared radiation in breast cancer therapy.
頁(從 - 到)1250-1262
期刊Journal of Proteome Research
出版狀態已發佈 - 2月 6 2015

ASJC Scopus subject areas

  • 生物化學
  • 化學 (全部)


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