TY - JOUR
T1 - Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis
AU - Isfort, Robert J.
AU - Robinson, Daniel
AU - Kung, Hsing Jien
PY - 1990/1/1
Y1 - 1990/1/1
N2 - A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (> 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.
AB - A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (> 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.
KW - DNA
KW - Herpesvirus
KW - Pulse-field electrophoresis
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U2 - 10.1016/0166-0934(90)90099-2
DO - 10.1016/0166-0934(90)90099-2
M3 - Article
C2 - 2157730
AN - SCOPUS:0025249168
SN - 0166-0934
VL - 27
SP - 311
EP - 317
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 3
ER -