Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells

W. A Y Tzong-Der; L. I N Hui-Yi; K. U O Daih-Huang; Shang Jie Tsai; Jia Ching Shieh; W. U. Jia-Chiun; L. E E Maw-Rong; L. I N Jen-Kun

doi:10.1021/jf900730e

Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells

W. A Y Tzong-Der, L. I N Hui-Yi, K. U O Daih-Huang, Shang Jie Tsai, Jia Ching Shieh, W. U. Jia-Chiun, L. E E Maw-Rong, L. I N Jen-Kun

研究成果: 雜誌貢獻 › 文章 › 同行評審

59 引文斯高帕斯（Scopus）

摘要

In the present study, we successively extracted the pu-erh raw tea with methanol (PR-1), chloroform (PR-2), ethyl acetate (PR-3), n-butanol (PR-4), and water (PR-5). Among these extracts, PR-3 extract contained ingredients with the most effective hypolipidemic potential and was further purified by column chromatography. Moreover, chronic administration of PR-3 provoked a significant reduction in levels of serum triglyceride and low-density lipoprotein (LDL) in rats. Our study demonstrated that fraction 5 from the PR-3 extract (PR-3-5s) showed a hypolipidemic effect in human hepatoma HepG2 cells. PR-3-5s decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) by stimulating AMP-activated protein kinase (AMPK) through the LKB1 pathway. Moreover, PR-3-5s blocked the progression of the cell cycle at the G1 phase by inducing p53 expression and in turn upregulating p21 expression.

原文	英語
頁（從 - 到）	5257-5264
頁數	8
期刊	Journal of Agricultural and Food Chemistry
卷	57
發行號	12
DOIs	https://doi.org/10.1021/jf900730e
出版狀態	已發佈 - 6月 24 2009
對外發佈	是

ASJC Scopus subject areas

農業與生物科學 (全部)
化學 (全部)

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10.1021/jf900730e

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Link to publication in Scopus

引用此

Tzong-Der, W. A. Y., Hui-Yi, L. I. N., Daih-Huang, K. U. O., Tsai, S. J., Shieh, J. C., Jia-Chiun, W. U., Maw-Rong, L. E. E., & Jen-Kun, L. I. N. (2009). Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells. Journal of Agricultural and Food Chemistry, 57(12), 5257-5264. https://doi.org/10.1021/jf900730e

Tzong-Der, WAY, Hui-Yi, LIN, Daih-Huang, KUO, Tsai, SJ, Shieh, JC, Jia-Chiun, WU, Maw-Rong, LEE & Jen-Kun, LIN 2009, 'Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells', Journal of Agricultural and Food Chemistry, 卷 57, 編號 12, 頁 5257-5264. https://doi.org/10.1021/jf900730e

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abstract = "In the present study, we successively extracted the pu-erh raw tea with methanol (PR-1), chloroform (PR-2), ethyl acetate (PR-3), n-butanol (PR-4), and water (PR-5). Among these extracts, PR-3 extract contained ingredients with the most effective hypolipidemic potential and was further purified by column chromatography. Moreover, chronic administration of PR-3 provoked a significant reduction in levels of serum triglyceride and low-density lipoprotein (LDL) in rats. Our study demonstrated that fraction 5 from the PR-3 extract (PR-3-5s) showed a hypolipidemic effect in human hepatoma HepG2 cells. PR-3-5s decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) by stimulating AMP-activated protein kinase (AMPK) through the LKB1 pathway. Moreover, PR-3-5s blocked the progression of the cell cycle at the G1 phase by inducing p53 expression and in turn upregulating p21 expression.",

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AU - Hui-Yi, L. I N

AU - Daih-Huang, K. U O

AU - Tsai, Shang Jie

AU - Shieh, Jia Ching

AU - Jia-Chiun, W. U.

AU - Maw-Rong, L. E E

AU - Jen-Kun, L. I N

PY - 2009/6/24

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N2 - In the present study, we successively extracted the pu-erh raw tea with methanol (PR-1), chloroform (PR-2), ethyl acetate (PR-3), n-butanol (PR-4), and water (PR-5). Among these extracts, PR-3 extract contained ingredients with the most effective hypolipidemic potential and was further purified by column chromatography. Moreover, chronic administration of PR-3 provoked a significant reduction in levels of serum triglyceride and low-density lipoprotein (LDL) in rats. Our study demonstrated that fraction 5 from the PR-3 extract (PR-3-5s) showed a hypolipidemic effect in human hepatoma HepG2 cells. PR-3-5s decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) by stimulating AMP-activated protein kinase (AMPK) through the LKB1 pathway. Moreover, PR-3-5s blocked the progression of the cell cycle at the G1 phase by inducing p53 expression and in turn upregulating p21 expression.

AB - In the present study, we successively extracted the pu-erh raw tea with methanol (PR-1), chloroform (PR-2), ethyl acetate (PR-3), n-butanol (PR-4), and water (PR-5). Among these extracts, PR-3 extract contained ingredients with the most effective hypolipidemic potential and was further purified by column chromatography. Moreover, chronic administration of PR-3 provoked a significant reduction in levels of serum triglyceride and low-density lipoprotein (LDL) in rats. Our study demonstrated that fraction 5 from the PR-3 extract (PR-3-5s) showed a hypolipidemic effect in human hepatoma HepG2 cells. PR-3-5s decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) by stimulating AMP-activated protein kinase (AMPK) through the LKB1 pathway. Moreover, PR-3-5s blocked the progression of the cell cycle at the G1 phase by inducing p53 expression and in turn upregulating p21 expression.

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Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells

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