TY - JOUR
T1 - Proton pump-rich cell secretes acid in skin of zebrafish larvae
AU - Lin, Li Yih
AU - Horng, Jiun Lin
AU - Kunkel, Joseph G.
AU - Hwang, Pung Pung
PY - 2006/2
Y1 - 2006/2
N2 - The mammalian kidney excretes its metabolic acid load through the proton-transporting cells, intercalated cells, in the distal nephron and collecting duct. Fish excrete acid through external organs, gill, or skin; however, the cellular function is still controversial. In this study, molecular and electrophysiological approaches were used to identify a novel cell type secreting acid in skin of zebrafish (Danio rerio) larvae. Among keratinocytes covering the larval surface, novel proton-secreting ionocytes, proton pump (H+-ATPase)-rich cells, were identified to generate strong outward H+ flux. The present work demonstrates for the first time, with a noninvasive technique, H+-secreting cells in an intact animal model, the zebrafish, showing it to be a suitable model in which to study the functions of vertebrate transporting epithelia in vivo.
AB - The mammalian kidney excretes its metabolic acid load through the proton-transporting cells, intercalated cells, in the distal nephron and collecting duct. Fish excrete acid through external organs, gill, or skin; however, the cellular function is still controversial. In this study, molecular and electrophysiological approaches were used to identify a novel cell type secreting acid in skin of zebrafish (Danio rerio) larvae. Among keratinocytes covering the larval surface, novel proton-secreting ionocytes, proton pump (H+-ATPase)-rich cells, were identified to generate strong outward H+ flux. The present work demonstrates for the first time, with a noninvasive technique, H+-secreting cells in an intact animal model, the zebrafish, showing it to be a suitable model in which to study the functions of vertebrate transporting epithelia in vivo.
KW - Epithelial transport
KW - Hydrogen-adenosinetriphosphatase
KW - Ion-selective electrode
KW - Ionocytes
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U2 - 10.1152/ajpcell.00281.2005
DO - 10.1152/ajpcell.00281.2005
M3 - Article
C2 - 16148031
AN - SCOPUS:33644867840
SN - 0363-6143
VL - 290
SP - C371-C378
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 2
ER -