TY - JOUR
T1 - Protective role of wogonin against lipopolysaccharide-induced angiogenesis via VEGFR-2, not VEGFR-1
AU - Lin, Chiu Mei
AU - Chang, Hang
AU - Chen, Yeh Hsu
AU - Li, Shih Yun
AU - Wu, I. Hsing
AU - Chiu, Jen Hwey
PY - 2006/11
Y1 - 2006/11
N2 - Wogonin, one of flavonoid derived from particular plants, enriches the property of anti-inflammation. Inflammation-stimulated angiogenesis plays an important role in many pathological diseases, such as rheumatoid arthritis, atherosclerosis, and cancer. The aim of this study was to investigate the suppressive effect of wogonin on lipopolysaccharide (LPS)-induced angiogenesis in human umbilical endothelial cell (HUVEC) cultures. By cell differentiation assays, migration and tube formation activity under LPS treatment were evaluated. Besides, IL-6 neutralizing antibody was added to test the inhibitory effect in the phenotypic alteration. Western blot analysis, ELISA cytokine assay, and quantitative real time-PCR were performed for VEGF, IL-6, VEGF receptors, and IL-6 receptor gene expressions on HUVEC with wogonin treatment. Furthermore, in vivo chorioallantoic membrane (CAM) assay was applied to evaluate the percentage of new vessels formation. The results revealed that wogonin (10- 8-10- 5 M) inhibited LPS-induced angiogenesis in a concentration-dependent manner. The mRNA and protein expressions of VEGF, VEGFR-2, IL-6, and sIL-6Rα were attenuated (P <0.05), but not VEGFR-1. In the LPS-induced CAM model, our data suggested that wogonin (10- 8-10- 5 M) significantly decreased new vessel formation and vascular network (P <0.05). We conclude that wogonin suppresses both in vitro and in vivo LPS-induced angiogenesis, through VEGFR-2, but not VEGFR-1.
AB - Wogonin, one of flavonoid derived from particular plants, enriches the property of anti-inflammation. Inflammation-stimulated angiogenesis plays an important role in many pathological diseases, such as rheumatoid arthritis, atherosclerosis, and cancer. The aim of this study was to investigate the suppressive effect of wogonin on lipopolysaccharide (LPS)-induced angiogenesis in human umbilical endothelial cell (HUVEC) cultures. By cell differentiation assays, migration and tube formation activity under LPS treatment were evaluated. Besides, IL-6 neutralizing antibody was added to test the inhibitory effect in the phenotypic alteration. Western blot analysis, ELISA cytokine assay, and quantitative real time-PCR were performed for VEGF, IL-6, VEGF receptors, and IL-6 receptor gene expressions on HUVEC with wogonin treatment. Furthermore, in vivo chorioallantoic membrane (CAM) assay was applied to evaluate the percentage of new vessels formation. The results revealed that wogonin (10- 8-10- 5 M) inhibited LPS-induced angiogenesis in a concentration-dependent manner. The mRNA and protein expressions of VEGF, VEGFR-2, IL-6, and sIL-6Rα were attenuated (P <0.05), but not VEGFR-1. In the LPS-induced CAM model, our data suggested that wogonin (10- 8-10- 5 M) significantly decreased new vessel formation and vascular network (P <0.05). We conclude that wogonin suppresses both in vitro and in vivo LPS-induced angiogenesis, through VEGFR-2, but not VEGFR-1.
KW - IL-6
KW - Lipopolysaccharide
KW - VEGF
KW - VEGFR-2
KW - Wogonin
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UR - http://www.scopus.com/inward/citedby.url?scp=33748421494&partnerID=8YFLogxK
U2 - 10.1016/j.intimp.2006.07.003
DO - 10.1016/j.intimp.2006.07.003
M3 - Article
C2 - 16979123
AN - SCOPUS:33748421494
SN - 1567-5769
VL - 6
SP - 1690
EP - 1698
JO - International Immunopharmacology
JF - International Immunopharmacology
IS - 11
ER -
