摘要
The expression of the chemokine, eotaxin-1, and its receptors in normal and osteoarthritic human chondrocytes was examined, and its role in cartilage degradation was elucidated in this study. Results indicated that plasma concentrations of eotaxin-1 as well as the chemokines, RANTES, and MCP-1α, were higher in patients with osteoarthritis (OA) than those in normal humans. Stimulation of chondrocytes with IL-1β or TNF-α significantly induced eotaxin-1 expression. The production of eotaxin-1 induced expression of its own receptor of CCR3 and CCR5 on the cell surface of chondrosarcomas, suggesting that an autocrine/paracrine pathway is involved in eotaxin-1's action. In addition, eotaxin-1 markedly increased the expressions of MMP-3 and MMP-13 mRNA, but had no effect on TIMP-1 expression in chondrocytes. However, pretreatment of anti-eotaxin-1 antibody significantly decreased the MMP-3 expression induced by IL-1β. These results first demonstrate that human chondrocytes express the chemokine, eotaxin-1, and that its expression is induced by treatment with IL-1β and TNF-α. The cytokine-triggered induction of eotaxin-1 further results in enhanced expressions of its own receptor of CCR3, CCR5, and MMPs, suggesting that eotaxin-1 plays an important role in cartilage degradation in OA.
原文 | 英語 |
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頁(從 - 到) | 929-939 |
頁數 | 11 |
期刊 | Journal of Cellular Biochemistry |
卷 | 93 |
發行號 | 5 |
DOIs | |
出版狀態 | 已發佈 - 2004 |
ASJC Scopus subject areas
- 生物化學
- 細胞生物學