TY - JOUR
T1 - Prevention of hepatic oxidative injury by Xiao-Chen-Chi-Tang in mice
AU - Tseng, Sung Hui
AU - Chien, Ting Yi
AU - Tzeng, Chih Fu
AU - Lin, Yun Ho
AU - Wu, Chih Hsiung
AU - Wang, Ching Chiung
PY - 2007/5/4
Y1 - 2007/5/4
N2 - The three purgative Cheng-Chi-Tang decoctions (CCTDs) including Ta-Cheng-Chi-Tang (TCCT), Xiao-Chen-Chi-Tang (XCCT), and Tiao-Wei-Chen-Chi-Tang (TWCCT) are used for treating gastrointestinal disorders, including liver diseases in traditional Chinese medicine. However, the underlying mechanisms as liver disease remedies are far from fully clarified. The objective of the study is to investigate and compare the antioxidant activity of the three purgative CCTDs in order to delineate their hepatic protective potential and mechanism. Antioxidant activity measured with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test indicated XCCT as the most potent preparation (IC50 8.94 μg/ml). In tert-butylhydroperoxide (TBH, 50 mM)-induced lipid peroxidation in ICR mice liver homogenates, XCCT also showed stronger and dose-dependent inhibitory activity against TBH-induced malondialdehyde (MDA, a marker of lipid peroxidation) production (IC50 53.66 μg/ml). In addition, XCCT showed dose-dependent protective effect against TBH-induced cytotoxicity in normal human Chung liver cells Furthermore, in carbon tetrachloride (CCl4)-induced acute liver injury model, mice pretreated with 0.2 g/kg and 0.4 g/kg of XCCT extracts showed a decrease of 59.8 and 43.1% in serum glutamic oxaloactetic transaminase (GOT) level, 51.4 and 52% in glutamic pyruvate transaminase (GPT) level, along with a reduction of 31 and 15% in MDA level, respectively, similar to the effects exerted by silymarin. XCCT pretreated mice also showed milder necrotic changes in the microscopic picture of the liver. The results suggest that XCCT has significant antioxidant activity and hepatic protection potential.
AB - The three purgative Cheng-Chi-Tang decoctions (CCTDs) including Ta-Cheng-Chi-Tang (TCCT), Xiao-Chen-Chi-Tang (XCCT), and Tiao-Wei-Chen-Chi-Tang (TWCCT) are used for treating gastrointestinal disorders, including liver diseases in traditional Chinese medicine. However, the underlying mechanisms as liver disease remedies are far from fully clarified. The objective of the study is to investigate and compare the antioxidant activity of the three purgative CCTDs in order to delineate their hepatic protective potential and mechanism. Antioxidant activity measured with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test indicated XCCT as the most potent preparation (IC50 8.94 μg/ml). In tert-butylhydroperoxide (TBH, 50 mM)-induced lipid peroxidation in ICR mice liver homogenates, XCCT also showed stronger and dose-dependent inhibitory activity against TBH-induced malondialdehyde (MDA, a marker of lipid peroxidation) production (IC50 53.66 μg/ml). In addition, XCCT showed dose-dependent protective effect against TBH-induced cytotoxicity in normal human Chung liver cells Furthermore, in carbon tetrachloride (CCl4)-induced acute liver injury model, mice pretreated with 0.2 g/kg and 0.4 g/kg of XCCT extracts showed a decrease of 59.8 and 43.1% in serum glutamic oxaloactetic transaminase (GOT) level, 51.4 and 52% in glutamic pyruvate transaminase (GPT) level, along with a reduction of 31 and 15% in MDA level, respectively, similar to the effects exerted by silymarin. XCCT pretreated mice also showed milder necrotic changes in the microscopic picture of the liver. The results suggest that XCCT has significant antioxidant activity and hepatic protection potential.
KW - Liver disease
KW - Oxidative injury
KW - Polygonaceae
KW - Purgative decoctions
KW - Rheum palmatum
KW - Xiao-Chen-Chi-Tang
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UR - http://www.scopus.com/inward/citedby.url?scp=34047099418&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2006.11.030
DO - 10.1016/j.jep.2006.11.030
M3 - Article
C2 - 17207593
AN - SCOPUS:34047099418
SN - 0378-8741
VL - 111
SP - 232
EP - 239
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 2
ER -