TY - JOUR
T1 - Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody
AU - Lin, Shin Fuw
AU - Coll, Miquel
AU - Gefter, Malcolm L.
AU - Margolies, Michael N.
AU - Wang, Andrew H.J.
N1 - Funding Information:
This work was supported by the National Institutes of Health (GM 37641 and CA 24432). M.C. is a Fulbright fellow.
PY - 1989/4/20
Y1 - 1989/4/20
N2 - Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do antizophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of β-octylglucoside as tetragonal bipyramids in the space group of either P41212 or P43312 and with unit cell dimensions of a = b = 77·9 A ̊, and c = 146·7 A ̊. They diffract X-rays to better than 2·7 Å resolution. Data up to 2·7 Å resolution have been collected.
AB - Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do antizophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of β-octylglucoside as tetragonal bipyramids in the space group of either P41212 or P43312 and with unit cell dimensions of a = b = 77·9 A ̊, and c = 146·7 A ̊. They diffract X-rays to better than 2·7 Å resolution. Data up to 2·7 Å resolution have been collected.
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U2 - 10.1016/0022-2836(89)90587-1
DO - 10.1016/0022-2836(89)90587-1
M3 - Article
C2 - 2738920
AN - SCOPUS:0024399697
SN - 0022-2836
VL - 206
SP - 789
EP - 790
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4
ER -