Post conductive keratoplasty visualization of rabbit cornea by multiphoton microscopy

Wen Lo, Tsung-Jen Wang, Fung Rong Hu, Chen Yuan Dong

研究成果: 書貢獻/報告類型會議貢獻


Conductive keratoplasty (CK) is a new refractive surgery for presbyopia and hyperopia patients. By applying radio frequency current at the peripheral regions of cornea, collagen, the most abundant composition of corneal stroma, shrinks due to the heat generated. The shrinkage at the periphery alters the corneal architecture and achieves clearer focus for near vision. In this work we use multiphoton microscopy to observe the post surgery structure variation at both submicron resolution and over a large region within the tissue. Since collagen can be induced to generate strong second harmonic generation (SHG) signal, multiphoton excitation provide direct visualization of collagen orientation within corneal stroma. In addition, since the SHG intensity of collagen tissue deteriorates with increasing thermal damage [1-3], our methodology can be used to characterize the extent of corneal stroma damage from the CK procedure. Finally, the influence of CK on the morphology and distribution of keratocytes can also be investigated by detecting multiphoton excited autofluorescence from the cells.
主出版物標題Optics InfoBase Conference Papers
發行者Optical Society of America
出版狀態已發佈 - 2007
事件European Conference on Biomedical Optics, ECBO 2007 - Munich, 德国
持續時間: 6月 17 20076月 17 2007


其他European Conference on Biomedical Optics, ECBO 2007

ASJC Scopus subject areas

  • 儀器
  • 原子與分子物理與光學


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